CAJ | 학술논문

目的探讨系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMCs)中磷脂酶C-γ1、磷脂酶C -γ2的表达水平及其与SLE疾病活动性的相关性.方法运用半定量聚合酶链反应(RT-PCR)法,检测30例SLE患者与25名健康对照人的磷脂酶C-γ1与磷脂酶C-γ2 mRNA表达水平,并采用Pearson相关性检验分析它们与补体C3、C4、抗双链DNA (dsDNA)抗体及SLE疾病活动指数(SLEDAI)积分的相关性.结果 ①SLE组与健康对照组相比,磷脂酶C-γ1与磷脂酶C-γ2 mRNA表达显著增高(分别为P＜0.01)；②磷脂酶C-γ1与磷脂酶C-γ2 mRNA表达水平之间呈正相关(r=0.726,P＜0.01)；③SLE组磷脂酶C-γ1 mRNA表达水平与补体C3、C4、抗dsDNA抗体均无相关性,而与SLEDAI积分呈正相关(r=0.002,P＜0.05).磷脂酶C-γ2与补体C3、C4呈显著负相关(r=-0.914,P＜0.01；r=-0.829,P＜0.05),与抗dsDNA抗体正相关(r=0.171,P＜0.05),与SLEDAI积分相关性不明显.结论本研究发现SLE患者磷脂酶C-γ1与磷脂酶C-γ2的表达水平在SLE患者中增高,磷脂酶C-γ1与SLEDAI积分呈正相关,磷脂酶C-γ2与补体C3、C4呈负相关,与抗dsDNA抗体正相关,可能对SLE患者的诊断和病情评估有较大价值.
목적 탐토계통성홍반랑창(SLE)환자외주혈단개핵세포(PBMCs)중린지매C-γ1、린지매C -γ2적표체수평급기여SLE질병활동성적상관성.방법 운용반정량취합매련반응(RT-PCR)법,검측30례SLE환자여25명건강대조인적린지매C-γ1여린지매C-γ2 mRNA표체수평,병채용Pearson상관성검험분석타문여보체C3、C4、항쌍련DNA (dsDNA)항체급SLE질병활동지수(SLEDAI)적분적상관성.결과 ①SLE조여건강대조조상비,린지매C-γ1여린지매C-γ2 mRNA표체현저증고(분별위P＜0.01)；②린지매C-γ1여린지매C-γ2 mRNA표체수평지간정정상관(r=0.726,P＜0.01)；③SLE조린지매C-γ1 mRNA표체수평여보체C3、C4、항dsDNA항체균무상관성,이여SLEDAI적분정정상관(r=0.002,P＜0.05).린지매C-γ2여보체C3、C4정현저부상관(r=-0.914,P＜0.01；r=-0.829,P＜0.05),여항dsDNA항체정상관(r=0.171,P＜0.05),여SLEDAI적분상관성불명현.결론 본연구발현SLE환자린지매C-γ1여린지매C-γ2적표체수평재SLE환자중증고,린지매C-γ1여SLEDAI적분정정상관,린지매C-γ2여보체C3、C4정부상관,여항dsDNA항체정상관,가능대SLE환자적진단화병정평고유교대개치.
Objective To investigate the expression levels of phospholipase C(PLC)-γ1 and PLC-γ2 in the peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus (SLE),and explore the relations between these genes expression levels and disease activity of SLE.Methods Reverse transcription polymerase chain reaction (RT-PCR) method was used to detect the expression levels of PLC-γ1 and PLC-γ2 in 30 patients with SLE and 25 controls.The associations between the expression levels of PLC- γ1 and PLC-γ2,complement C3,C4,antidouble stranded DNA antibody and SLEDAI scores in patients with SLE were analyzed by Pearson correlation analysis.Results ①The expression levels of PLC-γ1 and PLC-γ2 in the SLE patients were significantly higher than those of the normal controls (P＜0.01).) The expression levels of PLC-γ1 and PLC-γ2 showed positive correlations with each other (r=0.726,P＜0.01 ).③ The expression levels of PLC-γ2 were negatively correlated with serum complement C3,C4 (P＜0.05),but positively correlated with anti-double stranded DNA antibody,at the same time,they were not correlated with SLEDAI scores (P＞0.05).There was no correlation between complement C3,C4,anti-double stranded DNA antibody and the expression levels of PLC-γ1 (r=0.220,0.256,0.116,P＞0.05),but the expression levels of PLC-γ1 were positively correlated with SLEDAI scores.Conclusion We have shown that the expression levels of PLC-γ1 and PLC-γ2 is positively correlated and the PLC-γ1 and PLC-γ2 in patients with SLE are significantly higher than those of the normal controls.PLC-γ2 is negatively associated with complement C3,C4,PLC-γ1 is positively correlated with SLEDAI scores.Both PLC-γ1 and PLC-γ2 are be helpful in evaluating SLE disease activity and severity.