中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2010年
7期
800-803
,共4页
王艺婷%孙强正%刘凯%赵爱兰%王艳%金东%李振军%景怀琦%叶长芸%徐建国
王藝婷%孫彊正%劉凱%趙愛蘭%王豔%金東%李振軍%景懷琦%葉長蕓%徐建國
왕예정%손강정%류개%조애란%왕염%금동%리진군%경부기%협장예%서건국
福氏痢疾杆菌%血清型%前噬菌体%整合位点%排列方式
福氏痢疾桿菌%血清型%前噬菌體%整閤位點%排列方式
복씨이질간균%혈청형%전서균체%정합위점%배렬방식
Shigellaflexneri%Serotype%Prophage%Integration site%Arrangement
目的 研究福氏痢疾杆菌2b血清型菌株中SfII和SfX前噬菌体整合位点及排列方式.方法 根据2b血清型菌株基因组中SFII和SfX前噬菌体可能的整合位点及排列方式,设计引物,对50株2b血清型菌株进行PCR扩增,并对扩增产物测序,根据扩增和测序结果判断噬菌体的整合位点和排列方式.结果 在2b血清型菌株中,SfII和SfX前噬菌体前后相连,整合于宿主菌染色体proA和yaiC基因间的thrW tRNA基因上;以SfII在前,SfX在后,或SfX在前,SfII在后两种方式排列;而以后一种方式为主,在共50株检测菌株中,有49株为此方式.结论 福氏痢疾杆菌血清型相关噬菌体的整合位点及排列方式,对研究福氏痢疾杆菌血清型转换及血清型相关噬菌体的整合机制具有重要意义.
目的 研究福氏痢疾桿菌2b血清型菌株中SfII和SfX前噬菌體整閤位點及排列方式.方法 根據2b血清型菌株基因組中SFII和SfX前噬菌體可能的整閤位點及排列方式,設計引物,對50株2b血清型菌株進行PCR擴增,併對擴增產物測序,根據擴增和測序結果判斷噬菌體的整閤位點和排列方式.結果 在2b血清型菌株中,SfII和SfX前噬菌體前後相連,整閤于宿主菌染色體proA和yaiC基因間的thrW tRNA基因上;以SfII在前,SfX在後,或SfX在前,SfII在後兩種方式排列;而以後一種方式為主,在共50株檢測菌株中,有49株為此方式.結論 福氏痢疾桿菌血清型相關噬菌體的整閤位點及排列方式,對研究福氏痢疾桿菌血清型轉換及血清型相關噬菌體的整閤機製具有重要意義.
목적 연구복씨이질간균2b혈청형균주중SfII화SfX전서균체정합위점급배렬방식.방법 근거2b혈청형균주기인조중SFII화SfX전서균체가능적정합위점급배렬방식,설계인물,대50주2b혈청형균주진행PCR확증,병대확증산물측서,근거확증화측서결과판단서균체적정합위점화배렬방식.결과 재2b혈청형균주중,SfII화SfX전서균체전후상련,정합우숙주균염색체proA화yaiC기인간적thrW tRNA기인상;이SfII재전,SfX재후,혹SfX재전,SfII재후량충방식배렬;이이후일충방식위주,재공50주검측균주중,유49주위차방식.결론 복씨이질간균혈청형상관서균체적정합위점급배렬방식,대연구복씨이질간균혈청형전환급혈청형상관서균체적정합궤제구유중요의의.
Objective To study the integration site and arrangement of SfII and SfX prophages in Shigella flexneri serotype 2b strains. Methods A series of primers were designed based on potential integration site of SfII and SfX prophages in Shigella flexneri serotype 2b strains, and PCR were performed for 50 serotype 2b strains to amplify special genes located in host and prophages. PCR products were sequenced to identify integration sites and arrangement of SfII and SfX. Results In all the serotype 2b strains, prophage SfII and SfX were adjacent to each other, and integrated into the thrW tRNA gene of the host, which were located between genes proA and yaiC of host. Prophage SfX was located immediately upstream of prophage SfII in all the detected 50 serotype 2b strains exception for strain 51251. Conclusion This was the first report on the integration site and arrangement of serotype-converting prophages SfII and SfX in Shigella flexneri 2b strains.
