CAJ | 학술논문

目的构建乳腺癌中乳腺癌转移抑制基因1( BRMS1)反义基因重组腺病毒载体,并观察其对乳腺癌细胞MCF-7转移能力的影响。方法应用基因重组技术将BRMS1反义基因构建于腺病毒载体pAD-X,转染293包装细胞得到高滴度重组腺病毒,并用real-time聚合酶链反应(PCR)进行BRMS1基因表达的验证。将重组腺病毒pAD-aBRMS1感染MCF-7细胞后,应用Tran-°swell小室法观察对细胞侵袭和运动能力的影响。结果酶切结果与预期相符,real-time PCR可检测到感染BRMS1反义基因重组腺病毒后MCF-7细胞没有BRMS1基因表达。病毒转染后Transwell 小室可见,MCF-7细胞的侵袭和运动能力均受到显著的抑制(P值均＜0.01)。结论重组反义BRMS1腺病毒载体正确构建,其对乳腺癌细胞MCF-7的侵袭和运动都有抑制作用。
목적 구건유선암중유선암전이억제기인1( BRMS1)반의기인중조선병독재체,병관찰기대유선암세포MCF-7전이능력적영향。방법 응용기인중조기술장BRMS1반의기인구건우선병독재체pAD-X,전염293포장세포득도고적도중조선병독,병용real-time취합매련반응(PCR)진행BRMS1기인표체적험증。장중조선병독pAD-aBRMS1감염MCF-7세포후,응용Tran-°swell소실법관찰대세포침습화운동능력적영향。결과 매절결과여예기상부,real-time PCR가검측도감염BRMS1반의기인중조선병독후MCF-7세포몰유BRMS1기인표체。병독전염후Transwell 소실가견,MCF-7세포적침습화운동능력균수도현저적억제(P치균＜0.01)。결론 중조반의BRMS1선병독재체정학구건,기대유선암세포MCF-7적침습화운동도유억제작용。
Objective To construct breast cancer metastasis suppressor 1 (BRMS1) gene adenovirus vector and observe its effect on the metastasis ability of breast cancer cell line MCF-7. Methods BRMS1 gene was constructed into adenovirus vector pAD-X by gene recombination technique, which was transformed into 293 packaged cells for high titer adenovirus. Real-time polymerase chain reaction (PCR)was applied to detect BRMS1 gene expression. Transwell cabin assay was used to observe changes of invasion and motor ability of MCF-7 cells transfected with recombinant adenovirus. Results The finding of digestion was coincided with expected. No BRMS1 gene expression was detected in transfected MCF-7 cells by using real-time PCR. The invasion and motor abilities of transfected MCF-7 cells were significantly inhibited in transwell cabin assay (P ＜ 0. 01 ). Conclusion BRMS1 adenovirus was correctly reconstructed and it could inhibit invasion and motor abilities of transfected MCF-7 cells.