CAJ | 학술논문

目的对分离自慢性前列腺炎患者前列腺液中的一株棒状样细菌进行分类学鉴定,探讨其种系发生及生物学地位.方法利用形态、生理和生化特性等表型性状初步进行细菌鉴定,通过细胞壁化学成分分析、16S rRNA基因测序及基因比对明确其种系来源.结果分离、培养出革兰染色阳性的棒状样杆菌;生化反应不活泼;细胞壁二氨基酸为内消旋二氨基庚二酸(meso-DAP),全细胞糖含阿拉伯糖、半乳糖和甘露糖,化学型为胞壁Ⅳ型.GenBank数据库序列比对显示,该菌株与结核硬脂酸棒状杆菌(C tuberculostearicum)菌株ATCC35692的16S rDNA序列相似度最高,为99.4%(仅存在8个核苷酸差异).结论从表型性状和种系发生研究可确定该菌株为结核硬脂酸棒状杆菌,本研究与模式株相比亦存在一定差异,初步考虑该分离株可能足该菌种的一个新业型.该菌分离自前列腺液,在国内外尚属首例.
목적 대분리자만성전렬선염환자전렬선액중적일주봉상양세균진행분류학감정,탐토기충계발생급생물학지위.방법 이용형태、생리화생화특성등표형성상초보진행세균감정,통과세포벽화학성분분석、16S rRNA기인측서급기인비대명학기충계래원.결과 분리、배양출혁란염색양성적봉상양간균;생화반응불활발;세포벽이안기산위내소선이안기경이산(meso-DAP),전세포당함아랍백당、반유당화감로당,화학형위포벽Ⅳ형.GenBank수거고서렬비대현시,해균주여결핵경지산봉상간균(C tuberculostearicum)균주ATCC35692적16S rDNA서렬상사도최고,위99.4%(부존재8개핵감산차이).결론 종표형성상화충계발생연구가학정해균주위결핵경지산봉상간균,본연구여모식주상비역존재일정차이,초보고필해분리주가능족해균충적일개신업형.해균분리자전렬선액,재국내외상속수례.
Objective To carry out a taxonomic identification of a strain of claviform bacteria iso-lated from prostatic fluid of a patient who suffered from chronic prostatitis, and to approach its phylogenic and biologic position. Methods We undertaked an initial identification by phenotypic characters such as morphologecal, physiological and biochemical characteristics to ascertain its phylogeny by chemical composi-tion analysis of cell wall and 16S rRNA gene sequencing and alignment. Results A club-shaped gram posi-tive rod bacillus was isolated in pure culture state. Its biochemical reactions were not active. The diamino-acid of cell wall was meso-diaminopimelic acid (meso-DAP) and it had wall chemotype Ⅳ ( contained arabi-nose, galactose and maltose ). Sequence searches of the GenBank database revealed that this strain had a highest level of 16S rDNA sequence similarity (99.4%) to C. tuberculostearicurn strain ATCC35692 with only 8 nucleotides difference. Conclusion On the basis of phenotypic and phylngenetie analysis, it is rea-sonable to assign this strain to the species C. tuberculostearicum, and this is the first isolation of C. tubercu-lostearicum from prostatic fluid home and abroad.