中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2011年
1期
8-12
,共5页
耿晓星%全丽娜%马荣%唐丽萍
耿曉星%全麗娜%馬榮%唐麗萍
경효성%전려나%마영%당려평
宫颈肿瘤%As2O3%全反式维甲酸%HeLa细胞%N-myc下游调节基因1
宮頸腫瘤%As2O3%全反式維甲痠%HeLa細胞%N-myc下遊調節基因1
궁경종류%As2O3%전반식유갑산%HeLa세포%N-myc하유조절기인1
Uterine cervical neoplasms%Arsenic trioxide%All-trans retinoic acid%HeLa cell line%N-myc downstream regulated gene 1
目的 探讨As2O3和全反式维甲酸(ATRA)对人宫颈癌HeLa细胞体外生长的影响及其机制.方法 采用不同浓度As2O3和ATRA分别作用于人官颈癌HeLa细胞,观察细胞形态;采用四甲基偶氮唑蓝(MTT)比色法检测细胞生长情况;半定量逆转录聚合酶链反应(RT-PCR)和Westernblot法检测细胞中N-myc下游调节基因1(NDRG1)mRNA和蛋白的表达.结果 As2O3和ATRA能够抑制宫颈癌HeLa细胞生长,12.5 μmol/L As2O3处理HeLa细胞48 h的抑制率为(36.5±1.2)%,明显高于同时间低浓度组;1 × 10-5mol/LATRA处理HeLa细胞48 h的抑制率为(23.5±3.1)%,明显高于同时间低浓度组,并且同浓度的药物处理72 h的抑制率高于处理48 h的抑制率,呈浓度和时间依赖性(P<0.05).As2O3和ATRA能够从分子水平和蛋白水平上调宫颈癌HeLa细胞中NDRG1基因的表达,12.5 μmol/L As2O3和5×10-6mol/L ATRA处理HeLa细胞后,NDRG1 mRNA表达量分别为0.942±0.169和0.894±0.138,明显高于低浓度组的表达量;12.5 μmol/LAs2O3和5×10-6 mol/LATRA处理HeLa细胞后,NDRG1蛋白表达量分别为1.220±0.202和1.233±0.103,明显高于低浓度组的蛋白表达量.结论 As2O3能够抑制宫颈癌HeLa细胞生长,其抑制作用与NDRG1基因的表达上调相关.
目的 探討As2O3和全反式維甲痠(ATRA)對人宮頸癌HeLa細胞體外生長的影響及其機製.方法 採用不同濃度As2O3和ATRA分彆作用于人官頸癌HeLa細胞,觀察細胞形態;採用四甲基偶氮唑藍(MTT)比色法檢測細胞生長情況;半定量逆轉錄聚閤酶鏈反應(RT-PCR)和Westernblot法檢測細胞中N-myc下遊調節基因1(NDRG1)mRNA和蛋白的錶達.結果 As2O3和ATRA能夠抑製宮頸癌HeLa細胞生長,12.5 μmol/L As2O3處理HeLa細胞48 h的抑製率為(36.5±1.2)%,明顯高于同時間低濃度組;1 × 10-5mol/LATRA處理HeLa細胞48 h的抑製率為(23.5±3.1)%,明顯高于同時間低濃度組,併且同濃度的藥物處理72 h的抑製率高于處理48 h的抑製率,呈濃度和時間依賴性(P<0.05).As2O3和ATRA能夠從分子水平和蛋白水平上調宮頸癌HeLa細胞中NDRG1基因的錶達,12.5 μmol/L As2O3和5×10-6mol/L ATRA處理HeLa細胞後,NDRG1 mRNA錶達量分彆為0.942±0.169和0.894±0.138,明顯高于低濃度組的錶達量;12.5 μmol/LAs2O3和5×10-6 mol/LATRA處理HeLa細胞後,NDRG1蛋白錶達量分彆為1.220±0.202和1.233±0.103,明顯高于低濃度組的蛋白錶達量.結論 As2O3能夠抑製宮頸癌HeLa細胞生長,其抑製作用與NDRG1基因的錶達上調相關.
목적 탐토As2O3화전반식유갑산(ATRA)대인궁경암HeLa세포체외생장적영향급기궤제.방법 채용불동농도As2O3화ATRA분별작용우인관경암HeLa세포,관찰세포형태;채용사갑기우담서람(MTT)비색법검측세포생장정황;반정량역전록취합매련반응(RT-PCR)화Westernblot법검측세포중N-myc하유조절기인1(NDRG1)mRNA화단백적표체.결과 As2O3화ATRA능구억제궁경암HeLa세포생장,12.5 μmol/L As2O3처리HeLa세포48 h적억제솔위(36.5±1.2)%,명현고우동시간저농도조;1 × 10-5mol/LATRA처리HeLa세포48 h적억제솔위(23.5±3.1)%,명현고우동시간저농도조,병차동농도적약물처리72 h적억제솔고우처리48 h적억제솔,정농도화시간의뢰성(P<0.05).As2O3화ATRA능구종분자수평화단백수평상조궁경암HeLa세포중NDRG1기인적표체,12.5 μmol/L As2O3화5×10-6mol/L ATRA처리HeLa세포후,NDRG1 mRNA표체량분별위0.942±0.169화0.894±0.138,명현고우저농도조적표체량;12.5 μmol/LAs2O3화5×10-6 mol/LATRA처리HeLa세포후,NDRG1단백표체량분별위1.220±0.202화1.233±0.103,명현고우저농도조적단백표체량.결론 As2O3능구억제궁경암HeLa세포생장,기억제작용여NDRG1기인적표체상조상관.
Objective To study the effect of arsenic trioxide (As2O3 ) and all-trans retinoic acid ( ATRA ) on human cervical carcinoma HeLa cell line.Methods HeLa cells were treated with As2O3 and ATRA.The cell proliferation was evaluated by MTT assay.The expressions of NDRG-1 protein and mRNA were determined by Western blot and RT-PCR analysis.Results MTT assay showed that As2O3 and ATRA inhibited the growth of human cervical carcinoma HeLa cells in vitro in a dose- and time-dependent manner.Western blot and RT-PCR techniques showed that As2O3 and ATRA down-regulated the expressions of NDRG-1 protein and mRNA (P < 0.05 ) .Conclusion As2O3 and ATRA can significantly inhibit the growth and proliferation of HeLa cells.The reason of these changes may be related with the down-regulation of expression of NDRG-1.
