CAJ | 학술논문

建立了水产品中硝基呋喃类代谢物残留量测定的样品处理方法和高效液相色谱分析方法.样品经酸解、2.氯苯甲醛衍生后用乙酸乙酯萃取、SPE柱净化,经高效液相-紫外检测器测定.本方法4种硝基呋喃类代谢物的定量限均为1.0μg/kg,在5.0～500μg/L质量浓度范围内,4种硝基呋喃类代谢物的工作曲线均呈良好线性,在2个添加浓度水平的平均回收率为76%～102%,相对标准偏差均小于10%(n=6).该方法适用于定性定量水产品中硝基呋喃类代谢物的残留分析.
건립료수산품중초기부남류대사물잔류량측정적양품처리방법화고효액상색보분석방법.양품경산해、2.록분갑철연생후용을산을지췌취、SPE주정화,경고효액상-자외검측기측정.본방법4충초기부남류대사물적정량한균위1.0μg/kg,재5.0～500μg/L질량농도범위내,4충초기부남류대사물적공작곡선균정량호선성,재2개첨가농도수평적평균회수솔위76%～102%,상대표준편차균소우10%(n=6).해방법괄용우정성정량수산품중초기부남류대사물적잔류분석.
A quantitative high performance liquid chromatographic method using ultraviolet detection has been devel-oped for the determination of nitrofuran metabolites in aquatic products. The analytes were catalyzed under acidic con-ditions, and derivatized with 2-chlorobenzaldehyde (2-CPA). After liquid-liquid extraction with ethyl acetate and pu-rification with SPE column, identification and quantification of the analytes were done by high performance liquid chro-matography. The limits of quantification (LOQ) for AOZ, AMOZ, AHD, and SEM were 1.0 μg/kg. The calibration curves of the four compounds were linear in the concentration range of 5.0～500μg/L. The average recoveries of 2 spiked concentration levels were 76%～102% and the relative standard deviation of the method was less than 10% (n =6). The method was rapid, economical, and sensitive for identification and quantification of the nitrofuran me-tabolites in aquatic products.