CAJ | 학술논문

以早籼米为原料,用还原剂配合碱溶酸沉的方法提取大米蛋白.通过单因素试验与正交试验得出优化的提取条件为碱液浓度0.05mol/L、Na_2SO_3用量为米粉的0.5%、DTT用量为米粉的2.5%、固液比1:12(g/ml)、室温提取4h,在此条件下,大米蛋白提取率可达95.26%.
이조선미위원료,용환원제배합감용산침적방법제취대미단백.통과단인소시험여정교시험득출우화적제취조건위감액농도0.05mol/L、Na_2SO_3용량위미분적0.5%、DTT용량위미분적2.5%、고액비1:12(g/ml)、실온제취4h,재차조건하,대미단백제취솔가체95.26%.
In this study, a combinatorial method of reductant-alkali solution and acid precipitation was used to extract protein from rice endosperm. The optimal condition for rice protein extraction was determined through single factor and orthogonal experiments to be 0.05 mol/L sodium hydroxide, 0.50% sodium sulfite, 2.50% dithiothreitol, 1:12 (g/ml) of solid-liquid ratio, and 4 h of extraction time. Extraction rate of rice protein was 95.26% under this optimal condition.