中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2009年
49期
9735-9738
,共4页
李雷勇%田岳凤%王格洪%王军%张斌仁%王旭%孟颖霞
李雷勇%田嶽鳳%王格洪%王軍%張斌仁%王旭%孟穎霞
리뢰용%전악봉%왕격홍%왕군%장빈인%왕욱%맹영하
穴%神阙%穴%关元%穴%足三里%红细胞%CD58%艾灸%机体免疫
穴%神闕%穴%關元%穴%足三裏%紅細胞%CD58%艾灸%機體免疫
혈%신궐%혈%관원%혈%족삼리%홍세포%CD58%애구%궤체면역
背景:CD58是一种糖蛋白,属于免疫蛋白超家族成员,广泛表达于人体各种免疫细胞和红细胞,是红细胞调控T细胞免疫功能的重要天然免疫物质基础.目的:通过在神阙、关元等传统保健穴上施灸,观察对健康人群红细胞CD58表达的影响.设计、时间及地点:前后对照、调查分析.于2008-03/12在山西中医学院实验中心完成.对象:选择山西中医学院在校健康大学生40例,男女各20例,平均年龄21.08岁.受试者均知情同意.方法:将以熟地、山药、山茱萸等为主方的药物制成药饼置于受试者神阙、关元、足三里、脾俞、肾俞等穴施灸,隔日1次,共灸10次,进行施灸前后的对比.主要观察指标:在施灸前、后分别进行血细胞的常规检查,流式细胞仪测定红细胞表面CD58分子的阳性百分率和平均荧光强度.结果:施灸后红细胞、白细胞数量较施灸前显著升高(均为P<0.01);与施灸前相比,红细胞CD58分子的阳性百分率和平均荧光强度均明显高于施灸前(均为P<0.01).其中,红细胞CD58荧光强度的变化尤为突出.结论:红细胞CD58分子数量表达与艾灸密切相关,隔药饼灸增强了机体的免疫调节功能.
揹景:CD58是一種糖蛋白,屬于免疫蛋白超傢族成員,廣汎錶達于人體各種免疫細胞和紅細胞,是紅細胞調控T細胞免疫功能的重要天然免疫物質基礎.目的:通過在神闕、關元等傳統保健穴上施灸,觀察對健康人群紅細胞CD58錶達的影響.設計、時間及地點:前後對照、調查分析.于2008-03/12在山西中醫學院實驗中心完成.對象:選擇山西中醫學院在校健康大學生40例,男女各20例,平均年齡21.08歲.受試者均知情同意.方法:將以熟地、山藥、山茱萸等為主方的藥物製成藥餅置于受試者神闕、關元、足三裏、脾俞、腎俞等穴施灸,隔日1次,共灸10次,進行施灸前後的對比.主要觀察指標:在施灸前、後分彆進行血細胞的常規檢查,流式細胞儀測定紅細胞錶麵CD58分子的暘性百分率和平均熒光彊度.結果:施灸後紅細胞、白細胞數量較施灸前顯著升高(均為P<0.01);與施灸前相比,紅細胞CD58分子的暘性百分率和平均熒光彊度均明顯高于施灸前(均為P<0.01).其中,紅細胞CD58熒光彊度的變化尤為突齣.結論:紅細胞CD58分子數量錶達與艾灸密切相關,隔藥餅灸增彊瞭機體的免疫調節功能.
배경:CD58시일충당단백,속우면역단백초가족성원,엄범표체우인체각충면역세포화홍세포,시홍세포조공T세포면역공능적중요천연면역물질기출.목적:통과재신궐、관원등전통보건혈상시구,관찰대건강인군홍세포CD58표체적영향.설계、시간급지점:전후대조、조사분석.우2008-03/12재산서중의학원실험중심완성.대상:선택산서중의학원재교건강대학생40례,남녀각20례,평균년령21.08세.수시자균지정동의.방법:장이숙지、산약、산수유등위주방적약물제성약병치우수시자신궐、관원、족삼리、비유、신유등혈시구,격일1차,공구10차,진행시구전후적대비.주요관찰지표:재시구전、후분별진행혈세포적상규검사,류식세포의측정홍세포표면CD58분자적양성백분솔화평균형광강도.결과:시구후홍세포、백세포수량교시구전현저승고(균위P<0.01);여시구전상비,홍세포CD58분자적양성백분솔화평균형광강도균명현고우시구전(균위P<0.01).기중,홍세포CD58형광강도적변화우위돌출.결론:홍세포CD58분자수량표체여애구밀절상관,격약병구증강료궤체적면역조절공능.
BACKGROUND: CD58 is a glycoprotein, belongs to immune protein superfamily members, widely expresses in various human immunocytes and erythrocytes, and is the key immune substance of regulating T cell immune function by erythrocytes.OBJECTIVE: To explore the influence of moxibustion on the acupoints of Shenque (CV8) and Guanyuan (CV4) on erythrocyte CD58 expression in healthy people.DESIGN, TIME AND SETTING: A randomized, controlled experiment was performed at the Central Laboratory, Shanxi College of Traditional Chinese Medicine from March to December 2008.PARTICIPANTS: A total of 40 healthy undergraduates, 20 males and 20 females, aged mean 21.08 years, admitted in Shanxi College of Traditional Chinese Medicine were selected. Informed consents were obtained from each participant.METHODS: Putting the medicinal cakes on the acupoints, Shenque (CV8), Guanyuan (CV4), Zusanli (ST36), Pishu (BL20),Shenshu (BL21), and then lighted moxa every other day, totally for 10 times. Different quantities were compared before and after applying moxibustion.MAIN OUTCOME MEASURES: Hemocyte routine examination was performed before and after moxibustion. Positive percentage and mean fluorescent strength of CD58 molecule on erythrocyte surface were determined by flow cytometry.RESULTS: The quantities of erythrocytes and leukocytes were increased significantly following moxibustion (P < 0.01). Positive percentage and mean fluorescent strength of CD58 molecule on erythrocyte surface were significantly greater following moxibustion (P < 0.01), especially fluorescent strength of CD58 molecule on erythrocyte surface following moxibustion.CONCLUSION: There are close relationships between CD58 expression and moxibustion. Moxibustion with medicinal cake plays an important role in the immune function of a body.