CAJ | 학술논문

从白冠长尾雉粪样中分离出一株产氢量高的细菌菌株,命名为AUH-BG208.通过革兰氏染色和耐氧性测定,菌株AUH-BG208为革兰氏阴性兼性厌氧菌株.菌株AUH-BG208形态学及生理生化特征与克雷伯氏属一致,通过菌株16S rDNA序列分析,发现该菌株与肺炎克雷伯氏菌Klebsiella pneumoniae (AJ233420)相似性为100%.此外,对菌株AUH-BG208在不同条件下的产氢特性进行了测定.结果表明:该菌株在起始pH值为7.0,葡萄糖浓度为40 g/L,以酵母粉和硝酸钾为氮源时产氢能力最强,产氢量为1 900 mL/L.
종백관장미치분양중분리출일주산경량고적세균균주,명명위AUH-BG208.통과혁란씨염색화내양성측정,균주AUH-BG208위혁란씨음성겸성염양균주.균주AUH-BG208형태학급생리생화특정여극뢰백씨속일치,통과균주16S rDNA서렬분석,발현해균주여폐염극뢰백씨균Klebsiella pneumoniae (AJ233420)상사성위100%.차외,대균주AUH-BG208재불동조건하적산경특성진행료측정.결과표명:해균주재기시pH치위7.0,포도당농도위40 g/L,이효모분화초산갑위담원시산경능력최강,산경량위1 900 mL/L.
One bacterium designated AUH-BG208 with higher hydrogen-producing capacity was isolated from Syrmaticus reevesii feces. Bacterial strain AUH-BG208 is a facultative gram-negative bacterium based on gram-staining and oxygen-endurance ability test. Physiological and biochemical characteristics and morphological study showed that bacterial strain AUH-BG208 had the same properties of that of genus Klebsiella. BLAST search on the GenBank revealed that the full-length of 16S rDNA gene sequence of strain AUH-BG208 has 100% similarity to that of Klebsiella pneumoniae (AJ233420). Furthermore,hydrogen-producing capacity under different conditions of bacterium AUH-BG208 was studied as well. The results showed that the optimal conditions for strain AUH-BG208 were as follows:initial pH7.0,the concentration of glucose 40 g/L,nitrogen source mixture of yeast powder and potassium nitrate. The maximum hydrogen-producing yield was 1 900 mL/L under the optimal conditions.