中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2012年
7期
532-535
,共4页
徐波%陈海琛%黄国显%徐延波%武东珍
徐波%陳海琛%黃國顯%徐延波%武東珍
서파%진해침%황국현%서연파%무동진
褪黑激素%睾丸%再灌注损伤
褪黑激素%睪汍%再灌註損傷
퇴흑격소%고환%재관주손상
Melatonin%Testis%Reperfusion injury
目的 探讨褪黑激素对大鼠睾丸扭转的治疗作用.方法 选取青春期雄性SD大鼠48只,随机分为3组:空白对照组(A组)；扭转复位组(B组)；扭转复位+褪黑激素组(C组).B组和C组大鼠建立睾丸扭转复位模型,对照组不扭转.扭转4h后复位睾丸,复位前15 min B组腹腔注射生理盐水1 ml:C组腹腔注射褪黑激素1ml(17 mg/kg).复位后4h处死所有动物取睾丸待测.以原位缺口末端标记法(TUNEL)检测生精细胞凋亡指数；化学比色法测定睾丸组织内总抗氧化能力(T-AOC).结果 B组T-A0C( 20.31±2.55)U/mg比A组(33.62±3.29) U/mg明显降低,差异有统计学意义(P＜0.01).而C组T-AOC(30.05±2.08)U/mg较B组明显升高(P＜0.05).B组凋亡指数(42.2±3.21)％明显高于A组(5 7±0.67)％(P＜0.01),而C组凋亡指数(12.2±1.34)％较B组显著下降(P＜0.05).结论 褪黑激素具有明显对抗睾丸扭转复位后的氧化损伤,对因睾丸扭转导致的缺血再灌注损伤具有保护作用.
目的 探討褪黑激素對大鼠睪汍扭轉的治療作用.方法 選取青春期雄性SD大鼠48隻,隨機分為3組:空白對照組(A組)；扭轉複位組(B組)；扭轉複位+褪黑激素組(C組).B組和C組大鼠建立睪汍扭轉複位模型,對照組不扭轉.扭轉4h後複位睪汍,複位前15 min B組腹腔註射生理鹽水1 ml:C組腹腔註射褪黑激素1ml(17 mg/kg).複位後4h處死所有動物取睪汍待測.以原位缺口末耑標記法(TUNEL)檢測生精細胞凋亡指數；化學比色法測定睪汍組織內總抗氧化能力(T-AOC).結果 B組T-A0C( 20.31±2.55)U/mg比A組(33.62±3.29) U/mg明顯降低,差異有統計學意義(P＜0.01).而C組T-AOC(30.05±2.08)U/mg較B組明顯升高(P＜0.05).B組凋亡指數(42.2±3.21)％明顯高于A組(5 7±0.67)％(P＜0.01),而C組凋亡指數(12.2±1.34)％較B組顯著下降(P＜0.05).結論 褪黑激素具有明顯對抗睪汍扭轉複位後的氧化損傷,對因睪汍扭轉導緻的缺血再灌註損傷具有保護作用.
목적 탐토퇴흑격소대대서고환뉴전적치료작용.방법 선취청춘기웅성SD대서48지,수궤분위3조:공백대조조(A조)；뉴전복위조(B조)；뉴전복위+퇴흑격소조(C조).B조화C조대서건립고환뉴전복위모형,대조조불뉴전.뉴전4h후복위고환,복위전15 min B조복강주사생리염수1 ml:C조복강주사퇴흑격소1ml(17 mg/kg).복위후4h처사소유동물취고환대측.이원위결구말단표기법(TUNEL)검측생정세포조망지수；화학비색법측정고환조직내총항양화능력(T-AOC).결과 B조T-A0C( 20.31±2.55)U/mg비A조(33.62±3.29) U/mg명현강저,차이유통계학의의(P＜0.01).이C조T-AOC(30.05±2.08)U/mg교B조명현승고(P＜0.05).B조조망지수(42.2±3.21)％명현고우A조(5 7±0.67)％(P＜0.01),이C조조망지수(12.2±1.34)％교B조현저하강(P＜0.05).결론 퇴흑격소구유명현대항고환뉴전복위후적양화손상,대인고환뉴전도치적결혈재관주손상구유보호작용.
Objeetive To examine the protective effects of melatonin to testicular ischemiareperfusion injury caused by testicular torsion-torsion in rats.Methods Forty-eight adolescent male SD rats were randomly and evenly divided into 3 groups:A,control group; B,torsion-torsion group;C,torsion-torsion + melatonin treatment group.The rats of group B and C underwent a spennatic cord torsion lasting 4 hours.The rats of the control group were subjected to sham operation.In the group C rats,I ml melatonin (17mg/kg) was injected intraperitoneally 15 min before the cord was untwisted.The group B rats were injected with lml saline 4 hours after detorsion,all animals were sacrificed,and their testis were harvested for germ cell apoptosis (TUNEL) and testicular tissue total antioxidant capacity (T AOC) assay.Results T-AOC in the testis of group B rats decreased significantly compared with that of the A group rats (P<0.01 ),and T-AC in the testis of group C rats increased significantly compare to that of the group B rats (P<0.05).The apoptosis index in the testis of group B rats was significantly higher than that of group A rats (P<0.01 ),while the apoptotic index in the testis of group C rats decreased significantly compare to that of group B and C rats (P<0.05).Condusions Melatonin prevents testis from oxidative damage after testicular ischenia-reperfusion injury caused by testicular torsion in rats.
