CAJ | 학술논문

目的在肝特异性胰岛素样生长因子1(IGF-1)基因缺失(LID)小鼠及对照鼠体内建立原发性乳腺癌,应用基因芯片分析不同血清IGF-1水平下基因表达差异,探讨IGF-1与乳腺癌发生可能存在的关系.方法使用7,12-二甲基苯蒽(DMBA)在实验鼠体内诱导原发性乳腺癌,人参皂甙Rg3进行干预治疗.比较各组肿瘤发生情况并采用基因芯片比较各组基因差异表达情况.结果 4组小鼠乳腺癌发病率依次为66.67%、33.33%、36.00%、12.00%,肿瘤平均直径依次为(0.79±0.20)、(0.37±0.10)、(0.32±0.08)、(0.15±0.05)cm,4组小鼠血清IGF-1水平依次为(166.51±12.32)、(41.33±7.52)、(155.84±11.34)、(33.48±6.73)ng/ml.LID鼠与对照鼠相比,肿瘤潜伏期延长、发病率降低、瘤体生长缓慢.不同血清IGF-1水平下差异表达基因涉及代谢、凋亡、细胞周期、转录及翻译调控等多方面功能,其中Col11、Egln3、Glycaml、Irf6、Lgals7、Perp、Rag1、Rbm35a等基因可能与乳腺癌发病关系密切.结论 IGF-1可影响多基因表达而在乳腺癌发生中起促进作用,对相关基因的研究有助于认识乳腺癌的发病机制并为其治疗提供新的思路及实验依据.
목적 재간특이성이도소양생장인자1(IGF-1)기인결실(LID)소서급대조서체내건립원발성유선암,응용기인심편분석불동혈청IGF-1수평하기인표체차이,탐토IGF-1여유선암발생가능존재적관계.방법 사용7,12-이갑기분은(DMBA)재실험서체내유도원발성유선암,인삼조대Rg3진행간예치료.비교각조종류발생정황병채용기인심편비교각조기인차이표체정황.결과 4조소서유선암발병솔의차위66.67%、33.33%、36.00%、12.00%,종류평균직경의차위(0.79±0.20)、(0.37±0.10)、(0.32±0.08)、(0.15±0.05)cm,4조소서혈청IGF-1수평의차위(166.51±12.32)、(41.33±7.52)、(155.84±11.34)、(33.48±6.73)ng/ml.LID서여대조서상비,종류잠복기연장、발병솔강저、류체생장완만.불동혈청IGF-1수평하차이표체기인섭급대사、조망、세포주기、전록급번역조공등다방면공능,기중Col11、Egln3、Glycaml、Irf6、Lgals7、Perp、Rag1、Rbm35a등기인가능여유선암발병관계밀절.결론 IGF-1가영향다기인표체이재유선암발생중기촉진작용,대상관기인적연구유조우인식유선암적발병궤제병위기치료제공신적사로급실험의거.
Objective To investigate the possible relationship between liver-specific insulin-like growth factor I (IGF-1) and the pathogenesis of breast cancer.Methods Fifly non-IGF-l deficient (LID) mice were randomly divided into 2 equal groups:Group I,fed with dimethyl-benzanthracene (DMBA) for 8 weeks to cause mammary cancer,and Group Ⅲ,fed with DMBA and ginsenoside Rg for 28 d;and 50 LID mice were randomly divided into 2 equal groups too Group II,fed with DMBA for 8 weeks,and Group Ⅳ,fed with DMBA and ginsenoside Rg for 28 d.The mice were killed after the cessation of DMBA use.The serum IGF-1 expression was detected with method Six Gene chips were used to detect the gene expression in the breast cancer tissues.Results The breast cancer rates were 66.67% in Group I and 33.33% in Group Ⅱ.36.00% in Group Ⅲ,and 12.00% in Group Ⅳ.The tumor size was (0.79±0.20) cm in Group I,(O.37±0.08) cm in Group Ⅲ,(0.32±0.08) cm in Group II,and (0.15±0.05) cm Group Ⅳ.The IGF-1 level of Group Ⅱ was (41.33±7.52) ng/ml,1/4 as high as that of Group I[(166.51±12.32)ng/ml],and the IGF-1 level of Group Ⅳ was(33.48±6.73)ng/ml,1/4 as high as that of Group Ⅲ[(155.84±11.34)ng/mi].Compared with those of the control mice,tlle breast cancers of the LID mice had longer latency.10wer incidence,and slower growth rate.The differential gene expression in different serum IGF-1 levels involred binding,metabolism,apoptosis,cell eyele,signal transduction,immune response,transcription regulation and interpretation regulation and so on.Among these genes,Coll1,Egln3,Glycam1,Irf6,Lgals7,Perp,Ragl,and Rbm35a genes were closely related to the incidence of breast cancer.Condusion IGF-1 plays a role as a risk factor in the onset and development of breast cancer by affecting the expression of many dliferentially expressed genes.