中华医学遗传学杂志
中華醫學遺傳學雜誌
중화의학유전학잡지
CHINESE JOURNAL OF MEDICAL GENETICS
2010年
4期
423-427
,共5页
吕泽平%郑陈光%孔放%冯洁%江文宇%胡才友%黎华%吕渊%张根发%杨泽
呂澤平%鄭陳光%孔放%馮潔%江文宇%鬍纔友%黎華%呂淵%張根髮%楊澤
려택평%정진광%공방%풍길%강문우%호재우%려화%려연%장근발%양택
广西巴马人群%长寿%线粒体基因组
廣西巴馬人群%長壽%線粒體基因組
엄서파마인군%장수%선립체기인조
Bama population in Guangxi%longevity%mitochondrial genome
目的 探讨人类线粒体基因与广西巴马长寿的关联性.方法 对广西巴马96岁以上的20个老人外周血液中线粒体全序列进行测定,并与标准剑桥参考序列(Cambridge reference sequence,CRS)比对确定7个候选线粒体基因单核苷酸多态位点.用586名无亲缘关系的健康人群血样作为对照.并进一步用限制性片段长度多态性方法对候选基因进行检验.结果 线粒体DNA(mitochondrial DNA,mtDNA)4824A/G位点突变率在长寿和健康正常对照群体不同年龄段之间,随年龄增加突变频率逐渐提高,且对照人群样本突变率明显高于长寿人群,差异具有统计学意义.结论 mtDNA4824位点不仅是衰老相关位点,也是长寿负相关位点.
目的 探討人類線粒體基因與廣西巴馬長壽的關聯性.方法 對廣西巴馬96歲以上的20箇老人外週血液中線粒體全序列進行測定,併與標準劍橋參攷序列(Cambridge reference sequence,CRS)比對確定7箇候選線粒體基因單覈苷痠多態位點.用586名無親緣關繫的健康人群血樣作為對照.併進一步用限製性片段長度多態性方法對候選基因進行檢驗.結果 線粒體DNA(mitochondrial DNA,mtDNA)4824A/G位點突變率在長壽和健康正常對照群體不同年齡段之間,隨年齡增加突變頻率逐漸提高,且對照人群樣本突變率明顯高于長壽人群,差異具有統計學意義.結論 mtDNA4824位點不僅是衰老相關位點,也是長壽負相關位點.
목적 탐토인류선립체기인여엄서파마장수적관련성.방법 대엄서파마96세이상적20개노인외주혈액중선립체전서렬진행측정,병여표준검교삼고서렬(Cambridge reference sequence,CRS)비대학정7개후선선립체기인단핵감산다태위점.용586명무친연관계적건강인군혈양작위대조.병진일보용한제성편단장도다태성방법대후선기인진행검험.결과 선립체DNA(mitochondrial DNA,mtDNA)4824A/G위점돌변솔재장수화건강정상대조군체불동년령단지간,수년령증가돌변빈솔축점제고,차대조인군양본돌변솔명현고우장수인군,차이구유통계학의의.결론 mtDNA4824위점불부시쇠로상관위점,야시장수부상관위점.
Objective To investigate the human mitochondrial DNA (mtDNA) variations associated with longevity in Bama elderly population from Guangxi. Methods Mitochondrial genome of 20 individuals over 96 years of age was sequenced, and seven target single nucleotide polymorphism(SNPs) were observed by comparing with the standard rCRS sequence, and two were tested by polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) method in a larger population including 208 individuals of 90-113 years old, and 586 unrelated control individuals from Guangxi. Results The 4824G frequency of the mtDNA4824A/G locus increased with age both in the long-lived elderly and in controls.And it was significantly higher in controls than that in long-lived population (P＜0.05). Conclusion The mtDNA4824 A/G is not only an age-related locus, its mutation is also negatively correlated with longevity.
