CAJ | 학술논문

目的探讨蛋白激酶脑选择性激酶2(BRSK2)在胰腺导管腺癌中的表达情况及其与肿瘤的生物学行为之间的关系和对患者预后的影响.方法 79例胰腺导管腺癌组织标本取自复旦大学中山医院2005年至2007年获得手术切除且具有完整临床及随访资料的病例,利用免疫组织化学SABC方法对病理石蜡切片进行染色,对染色结果进行半定量分析,以3例正常胰腺组织中的染色情况作对照,观察BRSK2在胰腺癌及癌周正常组织中的表达情况.对所选病例按照肿瘤分化程度、TNM分期、有无血管、神经及淋巴结转移等进行分类,观察免疫组化结果与肿瘤生物学行为之间有无关联,并进一步分析BRSK2在肿瘤组织中的表达情况及对患者预后的影响.结果 BRSK2在正常胰腺组织中主要表达于胰岛以及小的导管如闰管、小叶内导管、小叶间导管等,但表达强度较弱;BRSK2在导管腺癌的癌周组织中表达亦较弱;BRSK2在79例胰腺导管腺癌中77例表达较强(++38例,+++39例);其表达与肿瘤有无淋巴结转移及远处转移、肿瘤的分期以及有无胰周神经浸润有关,在伴有淋巴结或远处转移、TNM晚期以及伴有胰周神经浸润的肿瘤中表达较强;而与肿瘤大小、分化及有无血管侵犯无关;BRSK2表达较强的肿瘤患者的预期生存期、中位生存期、1年和2年生存率均显著低于表达较弱的患者.结论 BRSK2在胰腺导管腺癌中表达上调,其表达强度与导管腺癌生物学行为之间存在显著相关,BRSK2在导管腺癌中的表达可能与患者的预后有关.
목적 탐토단백격매뇌선택성격매2(BRSK2)재이선도관선암중적표체정황급기여종류적생물학행위지간적관계화대환자예후적영향.방법 79례이선도관선암조직표본취자복단대학중산의원2005년지2007년획득수술절제차구유완정림상급수방자료적병례,이용면역조직화학SABC방법대병리석사절편진행염색,대염색결과진행반정량분석,이3례정상이선조직중적염색정황작대조,관찰BRSK2재이선암급암주정상조직중적표체정황.대소선병례안조종류분화정도、TNM분기、유무혈관、신경급림파결전이등진행분류,관찰면역조화결과여종류생물학행위지간유무관련,병진일보분석BRSK2재종류조직중적표체정황급대환자예후적영향.결과 BRSK2재정상이선조직중주요표체우이도이급소적도관여윤관、소협내도관、소협간도관등,단표체강도교약;BRSK2재도관선암적암주조직중표체역교약;BRSK2재79례이선도관선암중77례표체교강(++38례,+++39례);기표체여종류유무림파결전이급원처전이、종류적분기이급유무이주신경침윤유관,재반유림파결혹원처전이、TNM만기이급반유이주신경침윤적종류중표체교강;이여종류대소、분화급유무혈관침범무관;BRSK2표체교강적종류환자적예기생존기、중위생존기、1년화2년생존솔균현저저우표체교약적환자.결론 BRSK2재이선도관선암중표체상조,기표체강도여도관선암생물학행위지간존재현저상관,BRSK2재도관선암중적표체가능여환자적예후유관.
Objective To investigate the expression of BRSK2(brain selective kinase 2)in pancreatic ductal adenocarcinoma and to understand its clinical implication.Methods Resected tumor specimens of 79 pancreatic ductal adenocarcinoma were collected and paraffin-embedded for prepare.0.5μm sections.Immunohistochemical staining was employed to examine the expression pattern of BRSK2 translated protein.Semi-quantitative analysis was used to evaluate the intensity and content of protein expression in tumor tissues.The expression outcome was compared in peri-tumorous tissues and normal pancreatic tissues.Meanwhile,tumor samples were grouped according to their differentiation,TNM stage,with or without vascular or neural invasion.Then the possible relationship was explored between clinical,pathological and survival data and the expression profile of BRSK2 in tumor tissues.Results BRSK2's expression was weak in normal pancreatic tissues,including islets and minor ducts such as intercalated ducts,intralobular ducts and interlobular ducts.BRSK2's expression was also weak in peri-tumorous tissues.BRSK2's expression was strong in pancreatic ductal adenocarcinomns.It had a close relationship with lymphatic metastasis,distant metastasis,TNM staging as well as peri-pancreatic neural invasion.Tumors with lymphatic metastasis,distant metastasis and peri-pancreatic neural invasion or at later stages showed a higher expression of BRSK2 than those without or those at early stages.However the expression had no correlmion with tumor size,differentiation and vascular invasion.The expression of BBSK2 in pancreatic ductal adenocarcinonms was correlated with the prognosis of patients.Those with a higher expression pattern showed a shorter survival period.Conclusion BRSK2 is up-regulated in pancreatic ductal adenocarcinoma.And its expression is correlated with tumor biological behaviors and patient prognosis.