CAJ | 학술논문

背景:建立一种有效并简便可靠的高血压动物模型,对研究高血压的机制和治疗有重要的意义.目前,国内外常利用犬、大鼠、兔等实验制作高血压动物模型,其中肾动脉狭窄性高血压模型由于简便可靠被广泛应用于人类高血压及其并发症的研究.目的:建立简便、可靠的大鼠实验性肾动脉狭窄性高血压模型.设计:随机分组设计、动物实验.单位:青岛大学医学院附属医院脑血管病研究所.材料:实验于2005-09/2006-02在山东省脑病防治重点实验室完成.选择健康Wistar大鼠81只,随机数字表法分为单侧肾动脉狭窄组18只,双侧肾动脉狭窄组17只,单侧肾动脉结扎组15只,双侧肾动脉结扎组15只,单侧肾动脉狭窄假手术组6只,双侧肾动脉狭窄假手术组4只,正常对照组6只.方法:单侧肾动脉狭窄组,微型银夹夹闭右侧肾动脉,12 d后手术切除左侧肾脏.双侧肾动脉狭窄组,微型银夹夹闭右侧肾动脉,12 d后左侧做同样处理.单侧肾动脉结扎组,丝线结扎右侧肾动脉,12 d后手术切除左侧肾脏.双侧肾动脉结扎组,丝线结扎右侧肾动脉,12 d后左侧做同样处理.单侧肾动脉狭窄假手术组,手术暴露右侧肾脏,不做处理放回原位.12 d后切除左侧肾脏.双侧肾动脉狭窄假手术组,手术暴露右侧肾脏,不做处理放回原位,12 d后左侧做同样处理.正常对照组6只,不作任何处理.应用RBP-2大鼠血压计测量鼠尾血压和心率.主要观察指标:造模成功率、大鼠血压、心率.结果:纳入动物81只,死亡61只,均进入结果分析.①血压:单侧肾动脉狭窄组、双侧肾动脉狭窄组、双侧肾动脉结扎组血压明显高于正常对照组、双侧肾动脉狭窄假手术组[分别为(138.0±36.5),(154.2±11.6),(160.5±0.7),(101.3±17.6),(108.3±5.7)mm Hg].②心率:各肾动脉狭窄组动物心率变化不稳定,单侧肾动脉狭窄组、双侧肾动脉狭窄组、双侧肾动脉结扎组、正常对照组、双侧肾动脉狭窄假手术组分别为[(367.5±47.2),(420.2±47.8),(386.0±4.2),(390.3±42.4),(417.3±27.5)次/min].③存活率:肾动脉狭窄组存活率(单侧22%,双侧29%)显著高于肾动脉结扎组(单侧0,双侧12%),以双侧肾动脉狭窄组存活率最高.结论:双侧肾动脉夹闭法可建立稳定的肾动脉狭窄性高血压大鼠模型. 揹景:建立一種有效併簡便可靠的高血壓動物模型,對研究高血壓的機製和治療有重要的意義.目前,國內外常利用犬、大鼠、兔等實驗製作高血壓動物模型,其中腎動脈狹窄性高血壓模型由于簡便可靠被廣汎應用于人類高血壓及其併髮癥的研究.目的:建立簡便、可靠的大鼠實驗性腎動脈狹窄性高血壓模型.設計:隨機分組設計、動物實驗.單位:青島大學醫學院附屬醫院腦血管病研究所.材料:實驗于2005-09/2006-02在山東省腦病防治重點實驗室完成.選擇健康Wistar大鼠81隻,隨機數字錶法分為單側腎動脈狹窄組18隻,雙側腎動脈狹窄組17隻,單側腎動脈結扎組15隻,雙側腎動脈結扎組15隻,單側腎動脈狹窄假手術組6隻,雙側腎動脈狹窄假手術組4隻,正常對照組6隻.方法:單側腎動脈狹窄組,微型銀夾夾閉右側腎動脈,12 d後手術切除左側腎髒.雙側腎動脈狹窄組,微型銀夾夾閉右側腎動脈,12 d後左側做同樣處理.單側腎動脈結扎組,絲線結扎右側腎動脈,12 d後手術切除左側腎髒.雙側腎動脈結扎組,絲線結扎右側腎動脈,12 d後左側做同樣處理.單側腎動脈狹窄假手術組,手術暴露右側腎髒,不做處理放迴原位.12 d後切除左側腎髒.雙側腎動脈狹窄假手術組,手術暴露右側腎髒,不做處理放迴原位,12 d後左側做同樣處理.正常對照組6隻,不作任何處理.應用RBP-2大鼠血壓計測量鼠尾血壓和心率.主要觀察指標:造模成功率、大鼠血壓、心率.結果:納入動物81隻,死亡61隻,均進入結果分析.①血壓:單側腎動脈狹窄組、雙側腎動脈狹窄組、雙側腎動脈結扎組血壓明顯高于正常對照組、雙側腎動脈狹窄假手術組[分彆為(138.0±36.5),(154.2±11.6),(160.5±0.7),(101.3±17.6),(108.3±5.7)mm Hg].②心率:各腎動脈狹窄組動物心率變化不穩定,單側腎動脈狹窄組、雙側腎動脈狹窄組、雙側腎動脈結扎組、正常對照組、雙側腎動脈狹窄假手術組分彆為[(367.5±47.2),(420.2±47.8),(386.0±4.2),(390.3±42.4),(417.3±27.5)次/min].③存活率:腎動脈狹窄組存活率(單側22%,雙側29%)顯著高于腎動脈結扎組(單側0,雙側12%),以雙側腎動脈狹窄組存活率最高.結論:雙側腎動脈夾閉法可建立穩定的腎動脈狹窄性高血壓大鼠模型.
배경:건립일충유효병간편가고적고혈압동물모형,대연구고혈압적궤제화치료유중요적의의.목전,국내외상이용견、대서、토등실험제작고혈압동물모형,기중신동맥협착성고혈압모형유우간편가고피엄범응용우인류고혈압급기병발증적연구.목적:건립간편、가고적대서실험성신동맥협착성고혈압모형.설계:수궤분조설계、동물실험.단위:청도대학의학원부속의원뇌혈관병연구소.재료:실험우2005-09/2006-02재산동성뇌병방치중점실험실완성.선택건강Wistar대서81지,수궤수자표법분위단측신동맥협착조18지,쌍측신동맥협착조17지,단측신동맥결찰조15지,쌍측신동맥결찰조15지,단측신동맥협착가수술조6지,쌍측신동맥협착가수술조4지,정상대조조6지.방법:단측신동맥협착조,미형은협협폐우측신동맥,12 d후수술절제좌측신장.쌍측신동맥협착조,미형은협협폐우측신동맥,12 d후좌측주동양처리.단측신동맥결찰조,사선결찰우측신동맥,12 d후수술절제좌측신장.쌍측신동맥결찰조,사선결찰우측신동맥,12 d후좌측주동양처리.단측신동맥협착가수술조,수술폭로우측신장,불주처리방회원위.12 d후절제좌측신장.쌍측신동맥협착가수술조,수술폭로우측신장,불주처리방회원위,12 d후좌측주동양처리.정상대조조6지,불작임하처리.응용RBP-2대서혈압계측량서미혈압화심솔.주요관찰지표:조모성공솔、대서혈압、심솔.결과:납입동물81지,사망61지,균진입결과분석.①혈압:단측신동맥협착조、쌍측신동맥협착조、쌍측신동맥결찰조혈압명현고우정상대조조、쌍측신동맥협착가수술조[분별위(138.0±36.5),(154.2±11.6),(160.5±0.7),(101.3±17.6),(108.3±5.7)mm Hg].②심솔:각신동맥협착조동물심솔변화불은정,단측신동맥협착조、쌍측신동맥협착조、쌍측신동맥결찰조、정상대조조、쌍측신동맥협착가수술조분별위[(367.5±47.2),(420.2±47.8),(386.0±4.2),(390.3±42.4),(417.3±27.5)차/min].③존활솔:신동맥협착조존활솔(단측22%,쌍측29%)현저고우신동맥결찰조(단측0,쌍측12%),이쌍측신동맥협착조존활솔최고.결론:쌍측신동맥협폐법가건립은정적신동맥협착성고혈압대서모형.
BACKGROUND: It is significant to establish a kind of effective, conve nient and reliable animal model of hypertension. At present, dogs, rats and rabbits are usually used to establish hypertensive models at home and abroad, and the renal artery stenosis induced hypertensive models are ex tensively used to research hypertension and its complication for human be ings because they are convenient and reliable, and there are many methods to establish them, but the effects are to be evaluated. OBJECTIVE: To establish convenient and reliable animal models of ex perimental renal artery stenosis induced hypertension. DESIGN: A randomized grouping design and animal experiment. SETTING: Institute of Cerebrovascular diseases, Medical College Hospital of Qingdao University. MATERIALS: The experiments were carried out in Shandong Key Labora tory for Prevention and treatment of Brain Disease from September 2005 to February 2006. Eighty-one healthy Wistar rats divided into 7 groups accord ing to the method of random number table: unilateral renal artery stenosis group (n=18), bilateral renal artery stenosis group (n=17), unilateral renal artery ligation group (n=15), bilateral renal artery ligation group (n=15), uni lateral renal artery stenosis sham-operated group (n=6), bilateral renal artery stenosis sham-operated group (n=4) and normal control group (n=6). METHODS: Unilateral renal artery stenosis group: Right renal artery was clamped with miniature silver clip, and left kidney was resected after 12 days. Bilateral renal artery stenosis group: Right renal artery was clamped with miniature silver clip, and the same treatment was given to the left side after 12 days. Unilateral renal artery ligation group: Right renal artery was ligated with filament, and left kidney was resected after 12 days. Bilateral renal artery ligation group: Right renal artery was ligated with filament, and the same treatment was given to the left side after 12 days. Unilateral renal artery stenosis sham-operated group: Right kidney was exposed, and returned to the original place without any treatment, and left kidney was resected af ter 12 days. Bilateral renal artery stenosis sham-operated group: Right kid ney was exposed, and returned to the original place without any treatment, and the same treatment was given to the left side after 12 days. Normal con trol group: The rats were not given any treatment. The blood pressure and heart rate were determined with RBP-2 hemomanometer for rats. MAIN OUTCOME MEASURES: The successful rate of model estab lishment, blood pressure and heart rate were observed. RESULTS: Totally 81 rats were used, and 61 of them died, all were in volved in the analysis of results without deletion. ① Blood pressures in the unilateral and bilateral renal artery stenosis groups and bilateral renal artery ligation group were obviously higher than those in the normal control group and bilateral renal artery stenosis sham-operated group [(138.0 ±36.5), (154.2±11.6), (160.5±0.7), (101.3±17.6), (108.3±5.7) mm Hg]. ② The changes of heart rate in the renal artery stenosis group were unstable, and the heart rates in the unilateral and bilateral renal artery stenosis groups, bilateral renal artery ligation group, normal control group and bilat eral renal artery stenosis sham-operated group were (367.5±47.2), (420.2 ±47.8), (386.0±4.2), (390.3±42.4), (417.3±27.5) beats per minute, respec tively. ③ The survival rates in the renal artery stenosis groups (22%, 29%) were significantly higher than those in the renal artery ligation groups (0,12%), and it was the highest in the unilateral renal artery stenosis group.CONCLUSION: The method of clamping bilateral renal arteries can establish stable rat models of hypertension induced by renal artery stenosis.