CAJ | 학술논문

背景:有研究表明,在不同人群中,突变型54T FABP2与血脂障碍以及代谢综合征的其他特征相关.目的: 调查中老年人群小肠脂肪酸结合蛋白FABP2基因多态性频率分布,分析突变型54T FABP2基因与血脂水平的关系.设计:病例-对照分析.单位: 河北北方学院生物化学教研室和解放军第二五一医院检验科.对象:选择2003-10/2005-04在解放军第二五一医院体检中心进行体检的中老年人469名,男217名,平均年龄(56±10)岁;女252名,平均年龄(55±13)岁.除外肝、肾功能异常者,相互间无血缘关系;患者均对本实验均知情同意.实验已经医院伦理委员会批准.方法:①空腹12 h后,采用全自动生化仪(Olympus AU 6400)测定血浆总胆固醇、三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、载脂蛋白AⅠ、载脂蛋白B水平.②采集空腹静脉血1mL,枸橼酸钠抗凝,分离白细胞,蛋白酶K消化,酚/氯仿抽提基因组DNA.采用PCR-限制性片段长度多态性技术检测各组基因型分布频率.主要观察指标:①血脂水平.②FABP2 54位点基因型分布频率.结果:①频率分布:男性54A/T FABP2基因型频率分布为A/A0.48,A/T 0.42,T/T0.10;女性为A/A0.44,A/T0.46,T/T 0.10.男性和女性突变型54T等位基因频率分别为0.31,0.33.男女间频率分布情况比较,差异无显著性意义(P ＞ 0.05).②血脂:男性,54T等位基因携带者血浆低密度脂蛋白胆固醇、载脂蛋白B高于54A等位基因携带者,差异有显著性意义(P ＜ 0.05);女性,54T等位基因携带者血浆总胆固醇、低密度脂蛋白胆固醇高于54A等位基因携带者,差异有显著性意义(P ＜ 0.05).结论:在中老年人群中,FABP2基因多态性频率分布与性别无关;54T FABP2基因携带者有高血脂特征.
배경:유연구표명,재불동인군중,돌변형54T FABP2여혈지장애이급대사종합정적기타특정상관.목적: 조사중노년인군소장지방산결합단백FABP2기인다태성빈솔분포,분석돌변형54T FABP2기인여혈지수평적관계.설계:병례-대조분석.단위: 하북북방학원생물화학교연실화해방군제이오일의원검험과.대상:선택2003-10/2005-04재해방군제이오일의원체검중심진행체검적중노년인469명,남217명,평균년령(56±10)세;녀252명,평균년령(55±13)세.제외간、신공능이상자,상호간무혈연관계;환자균대본실험균지정동의.실험이경의원윤리위원회비준.방법:①공복12 h후,채용전자동생화의(Olympus AU 6400)측정혈장총담고순、삼선감유、고밀도지단백담고순、저밀도지단백담고순、재지단백AⅠ、재지단백B수평.②채집공복정맥혈1mL,구연산납항응,분리백세포,단백매K소화,분/록방추제기인조DNA.채용PCR-한제성편단장도다태성기술검측각조기인형분포빈솔.주요관찰지표:①혈지수평.②FABP2 54위점기인형분포빈솔.결과:①빈솔분포:남성54A/T FABP2기인형빈솔분포위A/A0.48,A/T 0.42,T/T0.10;녀성위A/A0.44,A/T0.46,T/T 0.10.남성화녀성돌변형54T등위기인빈솔분별위0.31,0.33.남녀간빈솔분포정황비교,차이무현저성의의(P ＞ 0.05).②혈지:남성,54T등위기인휴대자혈장저밀도지단백담고순、재지단백B고우54A등위기인휴대자,차이유현저성의의(P ＜ 0.05);녀성,54T등위기인휴대자혈장총담고순、저밀도지단백담고순고우54A등위기인휴대자,차이유현저성의의(P ＜ 0.05).결론:재중노년인군중,FABP2기인다태성빈솔분포여성별무관;54T FABP2기인휴대자유고혈지특정.
BACKGROUND: Studies have shown that alanine (A) to threonine (T) substitution at codon 54 of intestinal fatty acid-binding protein (FABP2) in different populations is associated with dyslipidemia and other characteristics of metabolic syndrome.OBJECTIVE: To investigate the frequency of encoding 54Ala/Thr (A/T) single nucleotide polymorphism in the FABP2 in middle-aged and old people, and explore the association between 54T FABP2 and plasma lipids.DESIGN: A case-controlled analysis. SETTING: Department of Biochemistry, Hebei North University and Department of Clinical Laboratory, the 251 Hospital of Chinese PLA.PARTICIPANTS: 469 physical examinees were selected from the Medical Examination Center, the 251 Hospital of Chinese PLA between October 2003 and April 2005. The subjects included 217 males with mean age of (56±10) years, and 252 females with mean age of (55±13) years. Only people with normal liver and kidney function, and with no blood relation were recruited. The informed consent to this study was obtained from all subjects. The experiment was admitted by Hospital Ethics Committee. METHODS: ①After fasting for 12 hours, automatic analyzer (Olympus AU 6400) was adopted to measure plasma total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), apoprotein A1(Apo A1) and Apo B levels. ②1 mL venous blood was extracted and immediately mixed with anti-coagulants containing citric acid, natrium citricum and glucose. White blood cells were separated and genomic DNA was isolated using standard methods with proteinase K digestion and phenol/chloroform purification. The genotype distribution frequency in each group was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). MAIN OUTCOME MEASURES: ①Plasma TC, TG, HDL-C, LDL-C, Apo A1and Apo B levels; ②Distributions of FABP2 genotypes at codon 54. RESULTS: ①The genotype frequencies of A/A, A/T, T/T were 0.48, 0.42, and 0.10 in males, and 0.44, 0.46, and 0.10 in females, respectively. The allelic frequency of point mutant 54Thr in FABP2 gene was 0.31 in males and 0.33 in females, respectively. There was no difference between males and females (χ2=0.47, P ＞ 0.05). ②The LDL-C and Apo B concentrations in fasting plasma of males with 54T allele were significantly higher than those with 54A allele (P ＜ 0.05). The TC and LDL-C concentrations in fasting plasma of females with 54T allele were significantly higher than those with 54A allele (P ＜ 0.05). CONCLUSION: In the middle-aged and old populations, the frequency of encoding 54Ala/Thr polymorphism in FABP2 gene is not correlated with gender, but with high lipoprotein profile.