CAJ | 학술논문

利用同源克隆的方法得到水稻的泛素连接酶APC/C辅助激活子CDH1的同源基因 OsCCS52B.通过蛋白质序列分析发现OsCCS52B 和苜蓿及拟南芥中的AtCCS52B(细胞周期转换开关基因)基因同源性最高;RNA原位杂交实验研究发现,OsCCS52B基因在减数分裂期间的表达存在一个高-低-高的波动变化.由于OsCCS52B表达变动的这个模式和减数分裂M-M(细胞分裂-细胞分裂)的转化过程中对受CDH1调控的细胞周期激酶CDKA活性的要求相一致,所以推测水稻的OsCCS52B基因参与了水稻减数分裂M-M转换期间对染色体复制的调控.同时,RNA原位杂交实验显示,OsCCS52B在核内复制旺盛的组织如根尖分生组织和穗下节的分生区和伸长区表达强烈,证明OsCCS52B可能参与了水稻的核内复制.
이용동원극륭적방법득도수도적범소련접매APC/C보조격활자CDH1적동원기인 OsCCS52B.통과단백질서렬분석발현OsCCS52B 화목숙급의남개중적AtCCS52B(세포주기전환개관기인)기인동원성최고;RNA원위잡교실험연구발현,OsCCS52B기인재감수분렬기간적표체존재일개고-저-고적파동변화.유우OsCCS52B표체변동적저개모식화감수분렬M-M(세포분렬-세포분렬)적전화과정중대수CDH1조공적세포주기격매CDKA활성적요구상일치,소이추측수도적OsCCS52B기인삼여료수도감수분렬M-M전환기간대염색체복제적조공.동시,RNA원위잡교실험현시,OsCCS52B재핵내복제왕성적조직여근첨분생조직화수하절적분생구화신장구표체강렬,증명OsCCS52B가능삼여료수도적핵내복제.
Through Blast research,OsCCS52B gene of the rice was identified as one of the CDH1 homologue.CDH1 is synonymously called the cell-cycle-switch (CCS52) in the plant.The deduced amino acid sequences and phylogenetic analysis revealed that OsCCS52B is the homologue of CDH1.RNA in situ hybridization showed that the expression of rice CDH1 homologue,OsCCS52B oscillates during meiosis.The oscillation of OsCCS52B matches the CDKA activity in meiosis,as CDKA is one of the targets of APC/CCDH1 an essential component in DNA replication.Therefore,we have proposed that OsCCS52B is involved in the control of chromosome replication during M-M(mitosis-mitosis) transition in rice.RNA in situ hybridization showed that OsCCS52B is highly expressed in the 2-day-old root tip.The result of this study suggests that OsCCS52B is also involved in the endoreduplication.