CAJ | 학술논문

建立了火焰原子吸收光谱法测定人血白蛋白中钾和钠的分析方法.样品中加入CsCl消除测定K、Na时的电离干扰,同时克服了试液中大量Na对测定K的干扰.由于样品中Na含量比较高,在测定时选用次灵敏线330.3 nm,可减少稀释倍数、节省试剂.对样品进行的消化处理和非消化处理对比试验表明:两种处理法测定结果无明显差异,但非消化处理的测定结果精密度较高(即RSD小),手续简便,只要将样品稀释后,加入CsCl,定容后即可进行测定.K、Na的检出限分别为0.077 8 mg/L,1.212 mg/L;特征浓度分别为0.033 mg/L(1%吸收), 2.864 mg/L(1%吸收); 方法的精密度(RSD, n=8)分别为 1.58%～1.63%和1.10%～1.16%, 样品加标回收率分别为98.6%～102.4%, 98.0%～101.1%.
건립료화염원자흡수광보법측정인혈백단백중갑화납적분석방법.양품중가입CsCl소제측정K、Na시적전리간우,동시극복료시액중대량Na대측정K적간우.유우양품중Na함량비교고,재측정시선용차령민선330.3 nm,가감소희석배수、절성시제.대양품진행적소화처리화비소화처리대비시험표명:량충처리법측정결과무명현차이,단비소화처리적측정결과정밀도교고(즉RSD소),수속간편,지요장양품희석후,가입CsCl,정용후즉가진행측정.K、Na적검출한분별위0.077 8 mg/L,1.212 mg/L;특정농도분별위0.033 mg/L(1%흡수), 2.864 mg/L(1%흡수); 방법적정밀도(RSD, n=8)분별위 1.58%～1.63%화1.10%～1.16%, 양품가표회수솔분별위98.6%～102.4%, 98.0%～101.1%.
The analysis is proposed for the determination of kalium and sodium in human serum albumin by flame atomic absorption spectrometry.CsCl is added to eliminate the ionization interference for the determination of kalium and sodium.A large amount of sodium is eliminated for the determination of kalium.The absorbing line of 330.3 nm is selected to determine sodium because of the high content of sodium in the samples.Dilution multiple is decreased and reagent is saved for the determination.The contrast examination is carried out under assimilation treatment and non-assimilation treatment condition.The examination results indicate that there is not a distinct difference in the analysis under the two conditions.The precision of the analysis result is better and the relative standard deviation(RSD) is smaller under the non-assimilation treatment condition. The samples are diluted to volume and CsCl is added. The detection limits are 0.077 8 mg/L for K and 1.212 mg/L for Na. The character concentrations are 0.033 mg/L(1% absorption) for K and 2.864 mg/L(1% absorption) for Na with precision (RSD,n=8) 1.58%-1.63% for K, 1.10%-1.16% for Na. The recoveries of the method are 98.6%-102.4% for K and 98.0%-101.6% for Na.