CAJ | 학술논문

对加拿大披碱草、老芒麦、杂种F1、C_0Ⅰ、C_1Ⅰ、C_0Ⅱ和C_1Ⅱ7种材料进行了同工酶和RAPD的分析,旨在通过分子标记方法探讨供试材料的亲缘关系.结果表明:在不同的生育期,EST同工酶酶谱具有较高的多态性,酶谱差异较大,在分蘖期杂种的EST酶谱偏向母本加拿大披碱草,根据该生育时期的酶谱特征可以鉴定杂种后代的的真实性,其他生育时期,EST酶带偏向父本老芒麦,而且没有明显的差异.SOD酶谱的差异只是体现在表达量的多少上,并没有出现特异性的条带.RAPD分析表明,加拿大披碱草与老芒麦的遗传一致度为0.72,遗传距离为0.328 5,在7种材料中亲缘关系最远;杂种后代与母本加拿大披碱草的遗传距离较近,而与父本老芒麦的亲缘关系相对较远;因此用RAPD分析材料的遗传关系更为合适.
대가나대피감초、로망맥、잡충F1、C_0Ⅰ、C_1Ⅰ、C_0Ⅱ화C_1Ⅱ7충재료진행료동공매화RAPD적분석,지재통과분자표기방법탐토공시재료적친연관계.결과표명:재불동적생육기,EST동공매매보구유교고적다태성,매보차이교대,재분얼기잡충적EST매보편향모본가나대피감초,근거해생육시기적매보특정가이감정잡충후대적적진실성,기타생육시기,EST매대편향부본로망맥,이차몰유명현적차이.SOD매보적차이지시체현재표체량적다소상,병몰유출현특이성적조대.RAPD분석표명,가나대피감초여로망맥적유전일치도위0.72,유전거리위0.328 5,재7충재료중친연관계최원;잡충후대여모본가나대피감초적유전거리교근,이여부본로망맥적친연관계상대교원;인차용RAPD분석재료적유전관계경위합괄.
Genetic analysis on isozymes and RAPD of E.canadensis,E.sibiricus,F1,C_0Ⅰ,C_1Ⅰ,C_0Ⅱand C_1Ⅱ were done.The results showed that the polymorphism of EST isozymes were much more than that of SOD during the four breeding periods. At tillering stage,the results of esterase isozymes also showed that true hybrid could be identificate,and zymogram characteristics of esterase isozymes tended to that of female parent E.canadensis. At other three breeding periods,zymogram characteristics of esterase isozymes tended to that of male parent and there were little differences . Differences of SOD isozyme only lied in expression level,and there were no specific band. Analysis on RAPD showed that genetic identity and genetic distance of E.canadensis and E.sibiricus were 0.72 and 0.328 5 . Their genetic relationship was most furthest among 7 materials.And genetic relationship between hybrids and parents showed that hybrids tended to female parent E.canadensis. But that was very far to male E.sibiricus.It was much appropriate that RAPD was used to analyze genetic relationship.