CAJ | 학술논문

研究了苗龄、外植体、激素配比对鞑靼荞麦(Fagopyrum tataricum Gaertn.)离体培养的影响,初步建立了鞑靼荞麦离体再生体系.结果表明,鞑靼荞麦离体再生的最佳取材时间为苗龄6～8 d;诱导愈伤组织的最适培养基为Ms+2.0 mg/L 2,4.D+1.5 mg/L 6-BA,子叶诱愈率达75%左右,下胚轴诱愈率可高达86.62%;愈伤组织分化的最适培养基为MS+0.1 mg/L IAA+2.0 mg/L 6-BA+1.0 mg/L K1.+0.5 mg/L TDZ,来自下胚轴愈伤组织的分化率可达9.52%.下胚轴的诱愈率与分化率均高于子叶,更适于离体再生培养.培养基巾加入AgNO_3后,能有效降低褐化率.生根最适培养基为含有0.5 mg/L NAA的1/2MS培养基,生根率在50%左右.TDZ在诱导鞑靼荞麦的愈伤组织分化出芽的过程中起到明显的促进作用,可提高分化率约20%.表3图2参23
연구료묘령、외식체、격소배비대달단교맥(Fagopyrum tataricum Gaertn.)리체배양적영향,초보건립료달단교맥리체재생체계.결과표명,달단교맥리체재생적최가취재시간위묘령6～8 d;유도유상조직적최괄배양기위Ms+2.0 mg/L 2,4.D+1.5 mg/L 6-BA,자협유유솔체75%좌우,하배축유유솔가고체86.62%;유상조직분화적최괄배양기위MS+0.1 mg/L IAA+2.0 mg/L 6-BA+1.0 mg/L K1.+0.5 mg/L TDZ,래자하배축유상조직적분화솔가체9.52%.하배축적유유솔여분화솔균고우자협,경괄우리체재생배양.배양기건가입AgNO_3후,능유효강저갈화솔.생근최괄배양기위함유0.5 mg/L NAA적1/2MS배양기,생근솔재50%좌우.TDZ재유도달단교맥적유상조직분화출아적과정중기도명현적촉진작용,가제고분화솔약20%.표3도2삼23
The effects of seedling age, hypocotyl and cotyledon as explants, and proportions of several growth regulators were tested in tissue culture of Fagopyrum tataricum Gaertn. for establishing its in vitro regeneration system. The results showed that the best seedling age for callus induction was 6 to 8 days. On the MS medium containing 2.0 mg/L 2, 4-D and 1.5 mg/L 6-BA, the induction rate of calli from hypocotyls was up to 86.62%, while that from cotyledons was about 75%. The suitable shooting medium was the MS medium+0.1 mg/L IAA+2.0 mg/L 6-BA+1.0 mg/L KT+0.5 mg/L TDZ (thidiazuron), and the shooting rate from hypocotyls was 9.52%. The callus induction and shooting rates were higher from hypocotyls than from cotyledons. Browning was reduced by the medium mixed with 3.5 mg/L AgNO_3 Half strength MS supplemented with 0.5 mg/L NAA was the best for rooting with a rate around 50% after 30 days' culture. TDZ could accelerate shoot differentiation distinctively, improving the shooting rate by nearly 20%. Fig 3, Tab 2, Ref 23