CAJ | 학술논문

目的:探讨胰岛素样生长因子结合蛋白相关蛋白1(insulin-like growth factor-binding protein related protein 1,IGFBP-rP1)基因在儿童急性白血病中的表达及临床意义.方法:应用实时荧光定量PCR (real-time fluorescence quantitative-PCR,RFQ-PCR )法检测168例(次)急性白血病患儿骨髓细胞中IGFBP-rP1 mRNA的表达水平,并与同期30例非白血病患儿作对照.根据儿童急性白血病的预后因素,分析IGFBP-rP1 mRNA的表达与患者临床预后的关系.结果: 初诊组急性白血病患儿的IGFBP-rP1 mRNA表达水平明显高于非白血病患儿(P<0.01).初诊组急性髓细胞白血病(acute myelocytic leukemia ,AML)患儿的IGFBP-rP1 mRNA表达水平高于急性淋巴细胞白血病(acute lymphoblastic leukemia ,ALL)患儿(P=0.013).完全缓解(complete remission,CR)组AML患儿的IGFBP-rP1 mRNA表达最低,接近非白血病患儿组;复发组AML患儿的IGFBP-rP1 mRNA表达水平再度上升,明显高于CR时的表达水平.而ALL患儿的IGFBP-rP1 mRNA表达水平在初诊组、CR组及复发组间的差异无统计学意义.结论:IGFBP-rP1基因可能参与儿童AML的发生和发展,并有望成为新的治疗靶点.
목적:탐토이도소양생장인자결합단백상관단백1(insulin-like growth factor-binding protein related protein 1,IGFBP-rP1)기인재인동급성백혈병중적표체급림상의의.방법:응용실시형광정량PCR (real-time fluorescence quantitative-PCR,RFQ-PCR )법검측168례(차)급성백혈병환인골수세포중IGFBP-rP1 mRNA적표체수평,병여동기30례비백혈병환인작대조.근거인동급성백혈병적예후인소,분석IGFBP-rP1 mRNA적표체여환자림상예후적관계.결과: 초진조급성백혈병환인적IGFBP-rP1 mRNA표체수평명현고우비백혈병환인(P<0.01).초진조급성수세포백혈병(acute myelocytic leukemia ,AML)환인적IGFBP-rP1 mRNA표체수평고우급성림파세포백혈병(acute lymphoblastic leukemia ,ALL)환인(P=0.013).완전완해(complete remission,CR)조AML환인적IGFBP-rP1 mRNA표체최저,접근비백혈병환인조;복발조AML환인적IGFBP-rP1 mRNA표체수평재도상승,명현고우CR시적표체수평.이ALL환인적IGFBP-rP1 mRNA표체수평재초진조、CR조급복발조간적차이무통계학의의.결론:IGFBP-rP1기인가능삼여인동AML적발생화발전,병유망성위신적치료파점.
Objective:To explore the expression of insulin-like growth factor-binding protein related protein 1(IGFBP-rP1) gene in children with acute leukemia and its potential significance. Methods:Real-time fluorescence quantitative PCR (RFQ-PCR) method was used for detecting IGFBP-rP1 mRNA expression in bone marrow (BM) cells of 168 children with acute leukemia. The results were compared with those of 30 non-leukemia children in control group. Meanwhile the relationship between IGFBP-rP1 expression level and clinical prognosis was analyzed according to clinical prognostic factors of children acute leukemia. Results:Expression level of IGFBP-rP1 in initial acute leukemia children was significantly higher than that of non leukemia children (P<0.01). It was higher in acute myeloid leukemia (AML) than in acute lymphoblastic leukemia (ALL)(P =0.013). The transcription level of IGFBP-rP1 mRNA in patients who had complete remission (CR) were lowest, which was nearly the same as non-leukemia childish patients. It increased again when leukemia relapsed, which was significantly higher than that in CR. However, as far as ALL was concerned, IGFBP-rP1 expression levels had no significant difference between newly-diagnosed, complete remission, and recurrent groups.Conclusion:IGFBP-rP1 may be involved in the initiation and development of childish leukemia. It has the potential to become a new target for AML treatment.