CAJ | 학술논문

研究了Pichia fermentans酵母菌产多糖YP5的单糖组成和结构.将酵母菌进行超声破碎后,采用乙醇沉淀法得到粗多糖,然后进行DEAE-纤维素色谱柱分离,得到均一的水溶性多糖YP5.经气相色谱分析表明,糖基由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖与半乳糖组成,其摩尔比为9.51∶ 1.33∶ 2.31∶ 0.94∶ 84.93∶ 0.96.乌氏粘度计法测得多糖的分子量为8.3×10~4.结合红外光谱和核磁共振1H NMR分析表明,该多糖YP5具有多糖特征性的红外吸收峰,存在酰胺结构,主要由β-D-葡聚糖组成.
연구료Pichia fermentans효모균산다당YP5적단당조성화결구.장효모균진행초성파쇄후,채용을순침정법득도조다당,연후진행DEAE-섬유소색보주분리,득도균일적수용성다당YP5.경기상색보분석표명,당기유서리당、아랍백당、목당、감로당、포도당여반유당조성,기마이비위9.51∶ 1.33∶ 2.31∶ 0.94∶ 84.93∶ 0.96.오씨점도계법측득다당적분자량위8.3×10~4.결합홍외광보화핵자공진1H NMR분석표명,해다당YP5구유다당특정성적홍외흡수봉,존재선알결구,주요유β-D-포취당조성.
The monosaccharide composition and the structure of polysaccharide from Pichia fermentans were analyzed. The yeast cells were broken with ultrasonic disruption and the crude polysaccharide was precipitated by addition of ethanol. Then, the crude polysaccharide was separated by diethylaminoethyl(DEAE) cellulose column separation and an acquisition of uniform water-soluble polysaccharide yeast polysaccharides 5(YP5) was obtained. The results of gas chromatographic analysis indicated that the glycosyl was made of rhamnose, arabinose, xylose, mannose, glucose and galactose, whose molar ratio was 9.51∶ 1.33∶ 2.31∶ 0.94∶ 84.93∶0.96, respectively. The molecular weight of polysaccharides YP5 was 8.3×10~4 measured by Ubbelohde viscosity method. Combination of IR and ~1H NMR analysis showed that the YP5 owned characteristic infrared absorption peaks of polysaccharide and contained amino groups. The β-D-glucan was main glucosidic linkage of polysaccharides PY5.