CAJ | 학술논문

目的:研究微小毛霉(HL-1)凝乳酶的酶学性质.方法:研究了酶的最适温度,酶的pH值稳定性和热稳定性,探讨了金属离子、化学物质和钙离了对酶活力的影响,加酶量对酶反应的影响,测定了酶的蛋白水解活力、相对分子量和酶的K_m与V_m值.结果:酶的最适温度为60℃; 最适pH值为5.5;酶在65℃保温5min活力损失95%;钙离子是酶的激活剂,其含量与酶活力正相关,而钾离子则对酶有抑制作用;氯化钠对酶活力的影响不大,但是SDS可导致酶严重失活;加酶量与反应时间成反比;酶的蛋白水解活力为485.3U/g;相对分子质量是42000,K_m为0.02380mol/L,V_m为1.1227mg/min.结论:得出了微小毛霉(HL-1)凝乳酶的酶学性质.
목적:연구미소모매(HL-1)응유매적매학성질.방법:연구료매적최괄온도,매적pH치은정성화열은정성,탐토료금속리자、화학물질화개리료대매활력적영향,가매량대매반응적영향,측정료매적단백수해활력、상대분자량화매적K_m여V_m치.결과:매적최괄온도위60℃; 최괄pH치위5.5;매재65℃보온5min활력손실95%;개리자시매적격활제,기함량여매활력정상관,이갑리자칙대매유억제작용;록화납대매활력적영향불대,단시SDS가도치매엄중실활;가매량여반응시간성반비;매적단백수해활력위485.3U/g;상대분자질량시42000,K_m위0.02380mol/L,V_m위1.1227mg/min.결론:득출료미소모매(HL-1)응유매적매학성질.
To study enzymatic properties of chymosin from Mucorpusillus(HL-1),the optimal temperature,pH and thermal stability of chymosin were investigated.Along with this the effects metal ions,chemical materials,calcium ions and enzyme amount on the activity of the enzyme wrrr examined,and the proteolytic activity,relalive molecular weight,K_m and V_m of the enzyme were measured.The optimal reaction temperature and pH of the enzyme was 60℃ and 5.5.Incubation at 65℃ for 5min resulted in 95%loss of enzyme activity.Calcium ion,an enzyme activator,could improve the enzyme activity in a concentrationdependent manner;in contrast,potassium ion was an inhibitor of the enzyme.Sodium chloride did not affect the enzyme activity,but SDS led to the severe inactivation of the enzyme.Meanwhile,a negative relationship between enzyme amount and reaction time was observed.The proteolytic activity,relative molecular mass,K_m and V_m of the enzyme were 485.3U/g,42000,0.0238 mol/L and 1.1227mg/min,respectively.