CAJ | 학술논문

[目的]为从分子水平进一步鉴定引起香蕉叶斑病的突脐蠕孢霉,对突脐蠕孢的Brn1基因序列进行了分析.[方法]用引物Brn1 01(5'-GCCAACATCGCAAACATGG-3’)and Brn1 02(5’-GCAAGCAGCACCGTCAATACCAAT-3')对3个分生孢子形态差异较大的香蕉突脐蠕孢叶斑病菌(CLER09 、D087和JL05)的Brn1基因进行PCR扩增和系统发育分析.[结果]不同菌株间存在简单的碱基缺失和替换,供试菌株与Exserohilum rostratum在系统发育树上聚类在同一个分支,与Exserohilum属其他种明显分开,形成一个独立的演化群.遗传距离分析表明,供试菌株与E.rostratum亲缘关系相近,菌株间的遗传距离为0.000～0.018,而Exserohilum属种间的遗传距离为0.020～0.093.[结论]E.rostratum菌株间的遗传变异较大,但仍属种内变异；Brn1基因可作为支持具有明显形态变异菌株为E.rostratum成员的分子依据.
[목적]위종분자수평진일보감정인기향초협반병적돌제연포매,대돌제연포적Brn1기인서렬진행료분석.[방법]용인물Brn1 01(5'-GCCAACATCGCAAACATGG-3’)and Brn1 02(5’-GCAAGCAGCACCGTCAATACCAAT-3')대3개분생포자형태차이교대적향초돌제연포협반병균(CLER09 、D087화JL05)적Brn1기인진행PCR확증화계통발육분석.[결과]불동균주간존재간단적감기결실화체환,공시균주여Exserohilum rostratum재계통발육수상취류재동일개분지,여Exserohilum속기타충명현분개,형성일개독립적연화군.유전거리분석표명,공시균주여E.rostratum친연관계상근,균주간적유전거리위0.000～0.018,이Exserohilum속충간적유전거리위0.020～0.093.[결론]E.rostratum균주간적유전변이교대,단잉속충내변이；Brn1기인가작위지지구유명현형태변이균주위E.rostratum성원적분자의거.
[Objective]Different strains of Exserohilum rostratum,causing exserohilum banana leaf spot disease were characterized at molecular level using the conserved sequence of Brn1 gene.[Method] Brn1 gene of three pathogenic isolates (CLER09,D087 and JL05) of E.rostratum causing exserohilum banana leaf spot disease was amplified using primer pair Brn1 01 (5'-GCCAACATCGCAAACATGG-3') and Brn1 02 (5'-GCAAGCAGCACCGTCAATACCAAT-3'),and phylogenetic analysis was carried out.[Result] The results showed absence and substitute of single bases in Brn1 sequences among different isolates of E.rostratum isolated from diseased banana plant and sequence collected from GenBank database.The isolates collected from banana were clustered with E.rostratum in an absolute evolution group in the phylogenetic tree,and significantly distinguished from other species of Exserohilum spp.Analysis result of the genetic distance matrix showed that tested isolates were genetically close to E.rostratum sequences collected from GenBank database with intraspecies genetic distance of 0.000-0.018 and interspecies genetic distance of 0.020-0.093 in Exserohilum genus.[ Conclusion ] The obvious genetic difference among isolates of E.rostratum could be considered as intraspecific variation and the Brn1 gene sequence may be used for molecular evidence for supporting the isolates with markedly morphological difference as members of E.rostratum.