CAJ | 학술논문

目的观察不同发育阶段黑胸大蠊水提取物对离体淋巴细胞增殖的影响. 方法提取小鼠脾细胞,制成一定浓度细胞悬液.实验分3组:对照组、伴刀豆球蛋白(Con A)阳性对照组和实验组.实验组设卵鞘、若虫、雌虫和雄虫等4组水提取物.每组设低、中、高剂量组,浓度依次为2.5 μg/ml、5.0μg/ml和10.0μg/ml,对照组加相同浓度的完全培养基.在一定条件下进行培养,再加入四甲基偶氮唑盐(methyl thiazolyl tetrazolium,MTT),测出各实验组570 nm波长的吸光度A值,运用方差分析进行统计学处理,以此判断淋巴细胞增殖情况;用吉氏染色法对各组淋巴细胞形态进行观察,通过其细胞体积、胞核及胞浆等的变化判断淋巴细胞增殖情况. 结果卵鞘、若虫、雌虫和雄虫的水提取物中高剂量组的,A值均高于对照组,若虫中剂量组和雌虫中、高剂量组A值依次为0.362±0.033、0.333±0.088、0.354±0.039,与对照组(0.231±0.020)比较,差异有统计学意义(P＜0.05),低剂量组内各实验组与对照组之间差异无统计学意义(P＞0.05);细胞形态学改变与A值变化一致. 结论黑胸大蠊若虫和雌虫水提取物在一定浓度时有促进离体淋巴细胞增殖的作用.
목적 관찰불동발육계단흑흉대렴수제취물대리체림파세포증식적영향. 방법 제취소서비세포,제성일정농도세포현액.실험분3조:대조조、반도두구단백(Con A)양성대조조화실험조.실험조설란초、약충、자충화웅충등4조수제취물.매조설저、중、고제량조,농도의차위2.5 μg/ml、5.0μg/ml화10.0μg/ml,대조조가상동농도적완전배양기.재일정조건하진행배양,재가입사갑기우담서염(methyl thiazolyl tetrazolium,MTT),측출각실험조570 nm파장적흡광도A치,운용방차분석진행통계학처리,이차판단림파세포증식정황;용길씨염색법대각조림파세포형태진행관찰,통과기세포체적、포핵급포장등적변화판단림파세포증식정황. 결과란초、약충、자충화웅충적수제취물중고제량조적,A치균고우대조조,약충중제량조화자충중、고제량조A치의차위0.362±0.033、0.333±0.088、0.354±0.039,여대조조(0.231±0.020)비교,차이유통계학의의(P＜0.05),저제량조내각실험조여대조조지간차이무통계학의의(P＞0.05);세포형태학개변여A치변화일치. 결론흑흉대렴약충화자충수제취물재일정농도시유촉진리체림파세포증식적작용.
Objective To observe the effect of aqueous extracts (E-Pf) from Periplaneta fuliginosa at different developmental stage on the lymphocyte proliferation in vitro. Methods The suspensions of mouse splenocyte were prepared and distributed into 3 groups: the control, the Con A treated positive control and the experiment (i.e. the E-Pf-treated ) groups, in which the experiment group was further divided into 4 sub-groups : the egg, the nymph, the male adult and the female adult E-Pf. The splenocyte suspension in each sub-group was cultured with high ( 10.0 μg/ml) , medium (5.0 μg/ml) and low ( 2.5 μg/ml) doses of its corre-sponding aqueous extract, respectively. The splenocyte suspension in the control group, however, was cultured merely with complete medium. Methyl thiazolyl tetrazolium (MTT) was then added into all groups, followed by the detection of optical density (A) values of MTT in each group. The proliferations of lymphocytes were judged by the statistical study of variance analysis. Morphological observations on the changes of cell volume, nucleus and cytoplasma were also done after Giemsa staining. Results The A values of all the middle- and high-dose E-Pf-treated subgroups were higher than that of the control group. The A values of the medium-dose nymph E-Pf ( 0.362 ± 0.033 ), the medium-dose (0.333 ± 0.088 ) and high-dose (0.354 ± 0.039 ) female adult E-Pf sub-groups were significantly higher than that of the control group (0.231 ± 0.020) ( P ＜ 0.05 ). No significant differences in A values were found between each of the low-dose E-Pf-treated subgroups and the control group ( P ＞ 0.05 ). The morphological changes of lymphocytes were consistent with the changes of A values. Con-clusion The Periplaneta fuliginosa aqueous extracts of nymph and adult females promote the lymphocyte prolif-eration in vitro.