中华外科杂志
中華外科雜誌
중화외과잡지
CHINESE JOURNAL OF SURGERY
2009年
3期
197-201
,共5页
郑召民%邝冠明%陈辉%吕游%张奎渤%刘辉%李佛保%董智勇
鄭召民%鄺冠明%陳輝%呂遊%張奎渤%劉輝%李彿保%董智勇
정소민%광관명%진휘%려유%장규발%류휘%리불보%동지용
组织工程%骨形态发生蛋白质类%基因修饰%脊柱融合术%腺相关病毒
組織工程%骨形態髮生蛋白質類%基因脩飾%脊柱融閤術%腺相關病毒
조직공정%골형태발생단백질류%기인수식%척주융합술%선상관병독
Tissue engineering%Bone morphogenetic proteins%Genetically modified%Spinal fusion%Adeno-associated virus
目的 探讨人骨形成蛋白4(hBMP-4)基因修饰的组织工程化骨促进兔脊柱融合的能力,为自体骨寻找理想的移植替代材料.方法 重组hBMP-4基因腺相关病毒(AAV-hBMP-4)和重组增强型绿色荧光蛋白(EGFP)基因腺相关病毒(AAV-EGFP)分别按实验确定的最佳感染复数(MOI)值转染兔骨髓间充质干细胞,复合Ⅰ型胶原海绵,构建出表达hBMP-4基因的组织工程化骨及表达对照基因EGFP的人工骨.采用新西兰兔后外侧脊柱融合模型,自身侧侧对照进行实验.14只实验动物随机分为2组,A组右侧为hBMP-4植入侧,左侧为自体骨对照侧.B组右侧为hBMP-4植入侧,左侧为EGFP对照侧.融合术后12周处死动物,评价不同移植物促进脊柱融合的情况.结果 术后2组各6只新西兰兔符合标准.术后第12周X线片、三维CT及扪诊检查示hBMP-4侧共11例达到骨性融合(11/12),自体骨侧5例融合(5/6),EGFP侧仅2例融合(2/6).大体标本观察hBMP-4及自体骨侧新生骨增生明显;EGFP侧成骨量少.结论 hBMP-4基因修饰的组织工程化骨在动物体内可以有效地促进成骨,提高脊柱融合率,可望成为一种理想的自体骨移植替代物.
目的 探討人骨形成蛋白4(hBMP-4)基因脩飾的組織工程化骨促進兔脊柱融閤的能力,為自體骨尋找理想的移植替代材料.方法 重組hBMP-4基因腺相關病毒(AAV-hBMP-4)和重組增彊型綠色熒光蛋白(EGFP)基因腺相關病毒(AAV-EGFP)分彆按實驗確定的最佳感染複數(MOI)值轉染兔骨髓間充質榦細胞,複閤Ⅰ型膠原海綿,構建齣錶達hBMP-4基因的組織工程化骨及錶達對照基因EGFP的人工骨.採用新西蘭兔後外側脊柱融閤模型,自身側側對照進行實驗.14隻實驗動物隨機分為2組,A組右側為hBMP-4植入側,左側為自體骨對照側.B組右側為hBMP-4植入側,左側為EGFP對照側.融閤術後12週處死動物,評價不同移植物促進脊柱融閤的情況.結果 術後2組各6隻新西蘭兔符閤標準.術後第12週X線片、三維CT及捫診檢查示hBMP-4側共11例達到骨性融閤(11/12),自體骨側5例融閤(5/6),EGFP側僅2例融閤(2/6).大體標本觀察hBMP-4及自體骨側新生骨增生明顯;EGFP側成骨量少.結論 hBMP-4基因脩飾的組織工程化骨在動物體內可以有效地促進成骨,提高脊柱融閤率,可望成為一種理想的自體骨移植替代物.
목적 탐토인골형성단백4(hBMP-4)기인수식적조직공정화골촉진토척주융합적능력,위자체골심조이상적이식체대재료.방법 중조hBMP-4기인선상관병독(AAV-hBMP-4)화중조증강형록색형광단백(EGFP)기인선상관병독(AAV-EGFP)분별안실험학정적최가감염복수(MOI)치전염토골수간충질간세포,복합Ⅰ형효원해면,구건출표체hBMP-4기인적조직공정화골급표체대조기인EGFP적인공골.채용신서란토후외측척주융합모형,자신측측대조진행실험.14지실험동물수궤분위2조,A조우측위hBMP-4식입측,좌측위자체골대조측.B조우측위hBMP-4식입측,좌측위EGFP대조측.융합술후12주처사동물,평개불동이식물촉진척주융합적정황.결과 술후2조각6지신서란토부합표준.술후제12주X선편、삼유CT급문진검사시hBMP-4측공11례체도골성융합(11/12),자체골측5례융합(5/6),EGFP측부2례융합(2/6).대체표본관찰hBMP-4급자체골측신생골증생명현;EGFP측성골량소.결론 hBMP-4기인수식적조직공정화골재동물체내가이유효지촉진성골,제고척주융합솔,가망성위일충이상적자체골이식체대물.
Objective To evaluate the efficacy of hBMP-4 gene modified tissue engineered bone graft in the enhancement of rabbit spinal fusion and find an ideal kind of substitute for the autograft bone. Methods Rabbit BMSCs were cultured and transfected with AAV-hBMP-4 using different MOI value. The optimal MOI value were determined by observing cell's morphology change. BMSCs were then transfected with AAV-hBMP-4 and AAV-EGFP respectively, following which the transfected cells were evenly suspended in a collagen sponge Ⅰ, and implanted to either side of the L5,6 intertransverse spaces posterolateral in the New Zealand rabbits to induce spinal fusion. Fourteen rabbits were randomly divided into 2 groups. Group 1: AAV-hBMP-4 transfected BMSCs in the fight side(hBMP-4 side) and autograft bone in the left side. Group 2: AAV-hBMP-4 transfected BMSCs in the right side(hBMP-4 side) and AAV-EGFP transfeeted BMSCs in the left side(EGFP side). Radiographs and three-dimensional CT of the spine, manual palpation, gross and histological examination of the fusion masses for all the animals were performed subsequent to animals having been sacrificed at 12 weeks after surgery. Results Evaluation has been taken in 12 New Zealand rabbits delivered into 2 groups which meet the criterion after operation. Eleven in 12 implemented sides involved hBMP-4 achieved bony fusion,to which 5 in 6 autografted sides was similar. But only 2 in 6 sides in EGFP-group achieved bony fusion meanwhile. Three-dimensional CT scan and palpation also evidenced the results. Bone formation was observed obviously on specimen both in hBMP-4 sides and autografted ones. EGFP-group also got bony integration, but the quantity was small. Conclusion Tissue-engineered bone graft constructed from application of hBMP-4 is a fine substitute for autograft. Effective enhancement of bony integration in spinal fusion surgery has been evidenced in vivo.