CAJ | 학술논문

微管蛋白(tubulin)是一蛋白质超家族,其中α-,β-微管蛋白是主要的微管蛋白,而γ-微管蛋白主要在微管组装中起作用. 我们利用蛋白质印迹和激光共聚焦技术研究了γ-微管蛋白在猪卵母细胞成熟、受精和活化中的分布. γ-微管蛋白存在于猪卵母细胞中,并且在减数分裂成熟各个时期的量保持不变. 它聚集在微管上,特别是中期纺锤体的两极和后末期的中板. 体外受精和孤雌活化后,γ-微管蛋白聚集在雌雄原核的周围.另外它也存在于精子的顶体帽和颈部.在早期卵裂中,γ-微管蛋白聚集在胚胎的细胞核周围.实验结果表明,γ-微管蛋白在猪卵母细胞、精子和胚胎的微管组装中起重要的调节作用,在猪受精过程中,精子和卵子都向受精卵贡献中心体物质.
미관단백(tubulin)시일단백질초가족,기중α-,β-미관단백시주요적미관단백,이γ-미관단백주요재미관조장중기작용. 아문이용단백질인적화격광공취초기술연구료γ-미관단백재저란모세포성숙、수정화활화중적분포. γ-미관단백존재우저란모세포중,병차재감수분렬성숙각개시기적량보지불변. 타취집재미관상,특별시중기방추체적량겁화후말기적중판. 체외수정화고자활화후,γ-미관단백취집재자웅원핵적주위.령외타야존재우정자적정체모화경부.재조기란렬중,γ-미관단백취집재배태적세포핵주위.실험결과표명,γ-미관단백재저란모세포、정자화배태적미관조장중기중요적조절작용,재저수정과정중,정자화란자도향수정란공헌중심체물질.
Tubulin is one superfamily of proteins. α- and β-tubulins are the major proteins of microtubules, while γ-tubulin plays a critical role in the nucleation of microtubules. Western blotting and immunofluorescent confocal microscopy were used to study the assembly and transformation of γ-tubulin during meiotic maturation, fertilization and parthenogenetic activation of pig oocytes. γ-Tubulin exists in the porcine oocytes and its quantity remains stable during meiotic maturation. It is located in the region where there is microtubule assembly, especially on the spindle poles at metaphase and middle plate or midbody of elongating spindle at anaphase and telophase. After in vitro fertilization and parthenogenetic activation, γ-tubulin concentrated around the male and female chromatin and pronuclei. It is also found to be localized in sperm acrosomal cap and the neck. During early cleavage, γ-tubulin distributes around the blastomere nuclei. The results suggest that γ-tubulin is a major regulator of microtubule assembly in porcine eggs and that both oocyte and sperm contribute centrosome materials to fertilized eggs.