CAJ | 학술논문

质谱技术已经成为目前蛋白质鉴定的重要工具.定量分析细胞内蛋白质组的动态变化,是当前研究蛋白质功能、揭示细胞生物机理、寻找疾病蛋白标记物和药物靶标的迫切需要.本文综述了基于质谱技术蛋白质定量的策略、方法和应用等方面近年来的进展,评述了几种蛋白质质谱定量方法的特点和应用潜力.
질보기술이경성위목전단백질감정적중요공구.정량분석세포내단백질조적동태변화,시당전연구단백질공능、게시세포생물궤리、심조질병단백표기물화약물파표적박절수요.본문종술료기우질보기술단백질정량적책략、방법화응용등방면근년래적진전,평술료궤충단백질질보정량방법적특점화응용잠력.
Mass spectrometry has become a powerful technique for proteomics analysis. It would be highly desirable to obtain exact quantitative values of each protein in a system, and these dynamic protein data obtained from cell can provide important information to study protein function, reveal the biological process and discover protein biomarker and drug target. Here, we report the development of quantitative mass spectrometry-based proteomics on strategy, method and application in recent years and further it is addressed for their characteristics and potentialities in protein analysis.