CAJ | 학술논문

以右旋磷霉素为唯一碳源进行固体初筛和液体复筛,获得生物转化能力较强的32株菌;再以杀卤虫模型筛选具有杀虫活性物质的菌株,得到一株具有较强杀虫活性的真菌FM94,通过对该菌的形态特征观察及ITS序列分析,鉴定为腾仓赤霉(Gibberella fujikuroi);初步考察其生物转化条件,结果表明在以右旋磷霉素为唯一碳源,初始pH值为3.5,转化培养第4d时转化产物杀卤虫活性最高.
이우선린매소위유일탄원진행고체초사화액체복사,획득생물전화능력교강적32주균;재이살서충모형사선구유살충활성물질적균주,득도일주구유교강살충활성적진균FM94,통과대해균적형태특정관찰급ITS서렬분석,감정위등창적매(Gibberella fujikuroi);초보고찰기생물전화조건,결과표명재이우선린매소위유일탄원,초시pH치위3.5,전화배양제4d시전화산물살서충활성최고.
Thirty-two strains were screened with the dextrofosfomysin as the sole carbon source.Insecticidal activity strains were screened by liquid bioassay with brine shrimp.The results showed that a strain named FM94 had high insecticidal activity.Its morphology and internal transcribed spacer (ITS) sequence were studied.The results indicated that the strain FM94 belonged to Gibberella fujikuroi.The optimum transformation condition that produced high insecticidal activity was obtained.Dextrofosfomysin used as the sole carbon source,the initial pH 3.5,the highest transformation was found after incubated 4 days.