CAJ | 학술논문

目的研究脐带源间充质干细胞(UC-MSC)细胞因子分泌特征及其对脐血源CD+34细胞的造血支持作用,并与骨髓源间充质干细胞(BM-MSC)相对照.方法 RT-PCR法柃测UC-MSC和BM-MSC细胞凶子分泌.将脐血CD+34细胞分别接种于UC-MSC或BM-MSC滋养层共培养5周,收集贴壁与非贴壁细胞接种于标准甲基纤维素培养基,计数克隆形成细胞(CFC).结果 UC-MSC表达SCF、LIF、M-CSF、Flt-3、IL-6、GM-CSF、G-CSF、SDF-1和VEGF mRNA,不表达IL-3 mRNA.与BM-MSC相比,二者表达细胞因子谱相似,但BM-MSC不表达GM-CSF和G-CSF mRNA.共培养5周计数CFC显示,BFU-E、GFU-GM和CFU-GEMM在CD+34细胞/UC-MSC(75.5±27.9,100.0±25.1,9.5±7.3)与CD+34细胞/BM-MSC(70.5±28.8,108.3±9.5,8.5±5.1)共培养体系差异无统计学意义(P＞0.05).结论 UC-MSC与BM-MSC具有相似的造血支持功能和细胞因子分泌谱.
목적 연구제대원간충질간세포(UC-MSC)세포인자분비특정급기대제혈원CD+34세포적조혈지지작용,병여골수원간충질간세포(BM-MSC)상대조.방법 RT-PCR법령측UC-MSC화BM-MSC세포흉자분비.장제혈CD+34세포분별접충우UC-MSC혹BM-MSC자양층공배양5주,수집첩벽여비첩벽세포접충우표준갑기섬유소배양기,계수극륭형성세포(CFC).결과 UC-MSC표체SCF、LIF、M-CSF、Flt-3、IL-6、GM-CSF、G-CSF、SDF-1화VEGF mRNA,불표체IL-3 mRNA.여BM-MSC상비,이자표체세포인자보상사,단BM-MSC불표체GM-CSF화G-CSF mRNA.공배양5주계수CFC현시,BFU-E、GFU-GM화CFU-GEMM재CD+34세포/UC-MSC(75.5±27.9,100.0±25.1,9.5±7.3)여CD+34세포/BM-MSC(70.5±28.8,108.3±9.5,8.5±5.1)공배양체계차이무통계학의의(P＞0.05).결론 UC-MSC여BM-MSC구유상사적조혈지지공능화세포인자분비보.
Objective To investigate the cytokine spectrum and henlatopoiesis-supportive function of umbilical cord derived mesdnchymal stem cells(UC-MSC),and compare with those of normal adult bone marrow derived mesenchymal stem cells(BM-MSC).Methods The mRNA of cytokine production of UC-MSC and BM-MSC were determined by reverse transcriptasc polymerase chain reaction(RT-PCR)analysis.To evaluate hematopoiesis supporting activity,cord blood(CB)CD+34 cells were co-cultured with UC-MSC or BM-MSC.Colony-forming cells(CFC)were determined after 5 weeks of culture.Results RT-PCR assay showed that UC-MSC had a cytokine spectrum very similar to that of BM-MSC.including expression of the mRNA ofstem cell factor,leukemia inhibitor factor,macrophage colony stimulating factor,Flt3-ligand,interleukin-6,vascular endothelial growth factor and stromal derived factor-1.but UC-MCS additionally expressed mRNA of granulocyte macrophage and granulocyte colony-stimulating factors.After co-culture with CD+34 cord blood cells for 5 weeks,no significant difference in CFC was observed between the CD+34 cells/UC-MSC and CD+34 cells/BM-MSC co-cultures (P＞0.05). Conclusion The cytokine spectrum and hematopoiesis-supponive function of UC-MSC ale similar with that of BM-MSC.