中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2011年
7期
407-410
,共4页
褚志强%张佳林%李晓航%钟嘉明%刘永锋
褚誌彊%張佳林%李曉航%鐘嘉明%劉永鋒
저지강%장가림%리효항%종가명%류영봉
肝移植%西罗莫司%他克莫司%T淋巴细胞,调节
肝移植%西囉莫司%他剋莫司%T淋巴細胞,調節
간이식%서라막사%타극막사%T림파세포,조절
Liver transplantation%Sirolimus%Tacrolimus%T-lymphocytes,regulatory
目的 探讨西罗莫司(SRL)和钙调磷酸酶抑制剂(CNI)对肝移植受者外周血中CD4+CD25high T淋巴细胞水平的影响.方法 排除肝移植远期移植肝功能异常的受者,将移植肝功能长期(超过2年)稳定的受者47例纳入研究,其中免疫抑制方案使用SRL者15例(SRL组),使用CNI(均为他克莫司)者32例(CNI组).以同期38名健康成人志愿者作为正常对照.使用流式细胞仪检测各组受试者外周血中单个核细胞CD4、CD25及Foxp3的表达水平,比较各组间外周血中CD4+CD25high调节性T淋巴细胞(Treg细胞)的差异.结果 与正常对照组相比,CNI组外周血淋巴细胞中CD4+ CD25high T淋巴细胞的比例显著减少(P<0.05),SRL组CD4+ CD25high T淋巴细胞的比例显著升高(P<0.05).SRL组、正常对照组和CNI组受试者外周血中CD4+ CD25high Foxp3+ Treg 细胞占CD4+ T淋巴细胞的比例依次降低,分别为1.88%(1.56%～2.60%)、1.15%(0.57%～1.48%)和0.84%(0.46%～1.45%),3组间两两比较,差异均有统计学意义(P<0.01或P<0.05).CD4+ CD25 high T淋巴细胞表达Foxp3的阳性率超过95%,CD4+ CD25 low T淋巴细胞表达Foxp3的阳性率低于20%,CD4+ CD25-T淋巴细胞不表达Foxp3.结论 SRL可促进肝移植受者外周血中Treg细胞水平的升高,而CNI可降低Treg细胞的水平.
目的 探討西囉莫司(SRL)和鈣調燐痠酶抑製劑(CNI)對肝移植受者外週血中CD4+CD25high T淋巴細胞水平的影響.方法 排除肝移植遠期移植肝功能異常的受者,將移植肝功能長期(超過2年)穩定的受者47例納入研究,其中免疫抑製方案使用SRL者15例(SRL組),使用CNI(均為他剋莫司)者32例(CNI組).以同期38名健康成人誌願者作為正常對照.使用流式細胞儀檢測各組受試者外週血中單箇覈細胞CD4、CD25及Foxp3的錶達水平,比較各組間外週血中CD4+CD25high調節性T淋巴細胞(Treg細胞)的差異.結果 與正常對照組相比,CNI組外週血淋巴細胞中CD4+ CD25high T淋巴細胞的比例顯著減少(P<0.05),SRL組CD4+ CD25high T淋巴細胞的比例顯著升高(P<0.05).SRL組、正常對照組和CNI組受試者外週血中CD4+ CD25high Foxp3+ Treg 細胞佔CD4+ T淋巴細胞的比例依次降低,分彆為1.88%(1.56%～2.60%)、1.15%(0.57%～1.48%)和0.84%(0.46%～1.45%),3組間兩兩比較,差異均有統計學意義(P<0.01或P<0.05).CD4+ CD25 high T淋巴細胞錶達Foxp3的暘性率超過95%,CD4+ CD25 low T淋巴細胞錶達Foxp3的暘性率低于20%,CD4+ CD25-T淋巴細胞不錶達Foxp3.結論 SRL可促進肝移植受者外週血中Treg細胞水平的升高,而CNI可降低Treg細胞的水平.
목적 탐토서라막사(SRL)화개조린산매억제제(CNI)대간이식수자외주혈중CD4+CD25high T림파세포수평적영향.방법 배제간이식원기이식간공능이상적수자,장이식간공능장기(초과2년)은정적수자47례납입연구,기중면역억제방안사용SRL자15례(SRL조),사용CNI(균위타극막사)자32례(CNI조).이동기38명건강성인지원자작위정상대조.사용류식세포의검측각조수시자외주혈중단개핵세포CD4、CD25급Foxp3적표체수평,비교각조간외주혈중CD4+CD25high조절성T림파세포(Treg세포)적차이.결과 여정상대조조상비,CNI조외주혈림파세포중CD4+ CD25high T림파세포적비례현저감소(P<0.05),SRL조CD4+ CD25high T림파세포적비례현저승고(P<0.05).SRL조、정상대조조화CNI조수시자외주혈중CD4+ CD25high Foxp3+ Treg 세포점CD4+ T림파세포적비례의차강저,분별위1.88%(1.56%～2.60%)、1.15%(0.57%～1.48%)화0.84%(0.46%～1.45%),3조간량량비교,차이균유통계학의의(P<0.01혹P<0.05).CD4+ CD25 high T림파세포표체Foxp3적양성솔초과95%,CD4+ CD25 low T림파세포표체Foxp3적양성솔저우20%,CD4+ CD25-T림파세포불표체Foxp3.결론 SRL가촉진간이식수자외주혈중Treg세포수평적승고,이CNI가강저Treg세포적수평.
Objective To investigate the possible influence of immunosuppressive therapy,including sirolimus (SRL) and calcineurin inhibitors (CNI, tacrolimus), on level of Treg in the liver allo-graft recipients, Methods Forty-seven liver transplant recipients with stable liver function were assessed for at least 2 years, and divided into two groups: one composed of 15 patients receiving SRL,and another, of those receiving CNI (32 patients with tacrolimus). Thirty-eight age-matched healthy subjects (HS) were used as normal controls. We examined the expression of CD4, CD25, and Foxp3 in peripheral blood monouclear cells. Treg of every group was analyzed using Cell Quest software.Results SRL significantly increased the prevalence of CD4+ CD25high T cells, compared with HS and CNI group. The prevalence of CD4+ CD25high T cells of CNIs group was significantly lower than that of HS. The percentage of CD4+ CD25high T cells in the total CD4+ T cells was 1.88 % (1.56 %-2. 60 %), 1. 15 % (0. 57 %-l. 48 %) and 0. 84 % (0. 46 %-1. 45 %) in SRL, NS and CNI group,respectively (P<0. 01 or P<0. 05). Foxp3 was expressed in more than 95 % of CD4 + CD25high T cells and less than 20 % of CD4+ CD25low T cells, whereas not expressed in CD4+ CD25- T cells.Conclusion Different immunosuppressive therapy (SRL or CNI) might have different roles in tolerance induction in liver transplant recipients. Namely, SRL promoted the induction of allo-antigen tolerance, but CNI hampered the progression.
