中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2010年
9期
860-863
,共4页
孙宏%耿文清%崔华露%赵敏%韩晓旭%张子宁%刘静%代娣%王亚男%姜拥军%尚红
孫宏%耿文清%崔華露%趙敏%韓曉旭%張子寧%劉靜%代娣%王亞男%薑擁軍%尚紅
손굉%경문청%최화로%조민%한효욱%장자저%류정%대제%왕아남%강옹군%상홍
HIV%AIDS%B细胞%Toll样受体
HIV%AIDS%B細胞%Toll樣受體
HIV%AIDS%B세포%Toll양수체
HIV%AIDS%B lymphocyte%Toll-like receptor
目的 探讨HIV-1感染后外周血B细胞数量的变化,以及B细胞TLR9 mRNA表达水平与HIV-1感染疾病进展的关系.方法 采集HIV/AIDS患者EDTA抗凝静脉血,荧光抗体染色后用流式细胞仪检测HIV/AIDS患者B细胞数量.密度梯度离心法分离外周血单个核细胞,应用MACS磁珠分选系统分选CD19+B细胞,并采用荧光定量实时PCR技术检测B细胞TLR9 mRNA水平.结果 HIV/AIDS患者B细胞数量显著低于健康对照组(P＜0.01),与CD4+T细胞数量呈正相关(r=0.534,P=0.006).HIV/AIDS患者外周血B细胞TIR9 mRNA的表达明显低于健康对照组(P=0.023),与CD4+T细胞数量呈正相关(r=0.390,P=0.040).结论 HIV感染可降低HIV/AIDS患者外周血B细胞数量以及B细胞TLR9 mRNA的表达量,B细胞数量和B细胞TLR9 mRNA的表达量均可能与疾病进展相关.
目的 探討HIV-1感染後外週血B細胞數量的變化,以及B細胞TLR9 mRNA錶達水平與HIV-1感染疾病進展的關繫.方法 採集HIV/AIDS患者EDTA抗凝靜脈血,熒光抗體染色後用流式細胞儀檢測HIV/AIDS患者B細胞數量.密度梯度離心法分離外週血單箇覈細胞,應用MACS磁珠分選繫統分選CD19+B細胞,併採用熒光定量實時PCR技術檢測B細胞TLR9 mRNA水平.結果 HIV/AIDS患者B細胞數量顯著低于健康對照組(P＜0.01),與CD4+T細胞數量呈正相關(r=0.534,P=0.006).HIV/AIDS患者外週血B細胞TIR9 mRNA的錶達明顯低于健康對照組(P=0.023),與CD4+T細胞數量呈正相關(r=0.390,P=0.040).結論 HIV感染可降低HIV/AIDS患者外週血B細胞數量以及B細胞TLR9 mRNA的錶達量,B細胞數量和B細胞TLR9 mRNA的錶達量均可能與疾病進展相關.
목적 탐토HIV-1감염후외주혈B세포수량적변화,이급B세포TLR9 mRNA표체수평여HIV-1감염질병진전적관계.방법 채집HIV/AIDS환자EDTA항응정맥혈,형광항체염색후용류식세포의검측HIV/AIDS환자B세포수량.밀도제도리심법분리외주혈단개핵세포,응용MACS자주분선계통분선CD19+B세포,병채용형광정량실시PCR기술검측B세포TLR9 mRNA수평.결과 HIV/AIDS환자B세포수량현저저우건강대조조(P＜0.01),여CD4+T세포수량정정상관(r=0.534,P=0.006).HIV/AIDS환자외주혈B세포TIR9 mRNA적표체명현저우건강대조조(P=0.023),여CD4+T세포수량정정상관(r=0.390,P=0.040).결론 HIV감염가강저HIV/AIDS환자외주혈B세포수량이급B세포TLR9 mRNA적표체량,B세포수량화B세포TLR9 mRNA적표체량균가능여질병진전상관.
Objective To study the B lymphocytes counts and the expression of TLR9 mRNA on B lymphocytes in peripheral blood from Chinese HIV-infected patients.Methods The cells from peripheral blood were stained with antibodies labeled with fluorescence and B lymphocytes were counted with flow cytometry.Using the method of magnetic activated cell sorting and real-time PCR,the expression of TLR9 mRNA was measured.Results The B lymphocytes counts in HIV/AIDS patients was significantly lower than that of healthy controls(P ＜0.01).The B lymphocytes counts in HIV/AIDS patients positively correlated with the CD4 +T cells counts(r =0.534,P = 0.006).The expression of TLR9 mRNA on B lymphocytes in HIV/AIDS patients was significantly lower than that of healthy controls(P =0.023),and positively correlated with the CD4 + T cells counts(r = 0.390,P = 0.040).Conclusion The B lymphocytes counts and the expression of TLR9 mRNA on B lymphocytes in Chinese HIV/AIDS patients were decreased due to HIV infection,which may correlate with disease progression.
