肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2010年
z1期
23-25
,共3页
卵巢肿瘤%基质金属蛋白酶-9%凋亡抑制基因Survivin
卵巢腫瘤%基質金屬蛋白酶-9%凋亡抑製基因Survivin
란소종류%기질금속단백매-9%조망억제기인Survivin
Ovarian neoplasms%Matrix metalloproteinase 9%Survivin
目的 探讨基质金属蛋白酶(MMP)-9、凋亡抑制基因Survivin与卵巢癌局部侵袭、发生、发展的关系.方法 收集正常上皮来源卵巢组织(n=20)、良性上皮性卵巢囊肿(n=30)、卵巢交界性肿瘤(n=11)和上皮性卵巢癌(n=64),采用免疫组织化学SP法进行定性分析,然后利用RT-PCR法检测Snrvivin和MMP-9基因的表达.结果 Survivin在正常卵巢组织、卵巢良性囊肿、卵巢交界性肿瘤及卵巢癌中阳性表达率分别为20.00%、20.00%、36.67%及76.56%,MMP-9在上述各组中阳性表达率分别为25.00%、23.33%、45.45%及79.68%;与正常卵巢组织、良性上皮性卵巢囊肿比较,卵巢癌组织中Survivin和MMP-9的表达增高明显(P<0.05).Survivin、MMP-9基因的表达分别为:正常卵巢组织(0.21±0.03)、(0.23±0.03);良性卵巢囊肿(0.19±0.05)、(0.21±0.05);卵巢交界性肿瘤(0.38±0.02)、(0.44±0.02);上皮性卵巢癌(0.58±0.02)、(0.59±0.04);与正常组比较均增加,良性上皮性卵巢囊肿、卵巢癌组织中Survivin和MMP-9的表达增高明显,差异有统计学意义(P<0.05).结论 卵巢肿瘤中Survivin和MMP-9的过度表达,促进了卵巢肿瘤的形成与发展.
目的 探討基質金屬蛋白酶(MMP)-9、凋亡抑製基因Survivin與卵巢癌跼部侵襲、髮生、髮展的關繫.方法 收集正常上皮來源卵巢組織(n=20)、良性上皮性卵巢囊腫(n=30)、卵巢交界性腫瘤(n=11)和上皮性卵巢癌(n=64),採用免疫組織化學SP法進行定性分析,然後利用RT-PCR法檢測Snrvivin和MMP-9基因的錶達.結果 Survivin在正常卵巢組織、卵巢良性囊腫、卵巢交界性腫瘤及卵巢癌中暘性錶達率分彆為20.00%、20.00%、36.67%及76.56%,MMP-9在上述各組中暘性錶達率分彆為25.00%、23.33%、45.45%及79.68%;與正常卵巢組織、良性上皮性卵巢囊腫比較,卵巢癌組織中Survivin和MMP-9的錶達增高明顯(P<0.05).Survivin、MMP-9基因的錶達分彆為:正常卵巢組織(0.21±0.03)、(0.23±0.03);良性卵巢囊腫(0.19±0.05)、(0.21±0.05);卵巢交界性腫瘤(0.38±0.02)、(0.44±0.02);上皮性卵巢癌(0.58±0.02)、(0.59±0.04);與正常組比較均增加,良性上皮性卵巢囊腫、卵巢癌組織中Survivin和MMP-9的錶達增高明顯,差異有統計學意義(P<0.05).結論 卵巢腫瘤中Survivin和MMP-9的過度錶達,促進瞭卵巢腫瘤的形成與髮展.
목적 탐토기질금속단백매(MMP)-9、조망억제기인Survivin여란소암국부침습、발생、발전적관계.방법 수집정상상피래원란소조직(n=20)、량성상피성란소낭종(n=30)、란소교계성종류(n=11)화상피성란소암(n=64),채용면역조직화학SP법진행정성분석,연후이용RT-PCR법검측Snrvivin화MMP-9기인적표체.결과 Survivin재정상란소조직、란소량성낭종、란소교계성종류급란소암중양성표체솔분별위20.00%、20.00%、36.67%급76.56%,MMP-9재상술각조중양성표체솔분별위25.00%、23.33%、45.45%급79.68%;여정상란소조직、량성상피성란소낭종비교,란소암조직중Survivin화MMP-9적표체증고명현(P<0.05).Survivin、MMP-9기인적표체분별위:정상란소조직(0.21±0.03)、(0.23±0.03);량성란소낭종(0.19±0.05)、(0.21±0.05);란소교계성종류(0.38±0.02)、(0.44±0.02);상피성란소암(0.58±0.02)、(0.59±0.04);여정상조비교균증가,량성상피성란소낭종、란소암조직중Survivin화MMP-9적표체증고명현,차이유통계학의의(P<0.05).결론 란소종류중Survivin화MMP-9적과도표체,촉진료란소종류적형성여발전.
Objective To detect the expression of MMP-9 and Survivin in ovary cancer and investigate their relationship in local invasion, genesis and development of ovarian cancer. Methods The tissues samples from 64 cases of epithelial ovarian cancer,30 cases of carcinoid, 11 cases of borderline tumor and 20 cases of normal were collected, expressions of MMP-9 and Survivin were detected by immuohistochemistry SP method and RT-PCR. Results The expression positive rates of Survivin were 20.00 %, 20.00 %, 36.67 % and 76.56 %, and the positive rates of MMP-9 were 25.00 %, 23.33 %, 45.45 % and 79.68 % in normal ovarian tissues, carcinoid, borderline tumor and epithelial cancer of ovary, respectively. The positive rates of MMP-9 and Survivin were were higher in ovary epithelial cancer carcinoid than those in carcinoid and normal ovarian tissues(P <0.05). The mRNA of Survivin were (0.21 ±0.03), (0.19± 0.05), (0.38±0.02) and (0.58±0.02), and the mRNA of MMP-9 were (0.23±0.03), (0.21±0.05), (0.44±0.02) and (0.59±0.04) in normal ovarian tissues, carcinoid, borderline tumor and epithelial cancer of ovary, respectively.The mRNA levels of Survivin and MMP-9 were higher in carcinoid and epithelial cancer of ovary than those in normal ovarian tissues. Conclusion The over expressions of Survivin and MMP-9 are related to genesis and development of ovarian cancer.
