CAJ | 학술논문

目的通过病例对照研究,了解内皮细胞蛋白C受体(EPCR)基因6936A/G多态性和深静脉血栓形成(DVT)的相关性,进一步了解EPCR在DVT形成中的重要性.方法用ELISA法检测65例DVT患者和71名健康体检者的外周血血浆可溶性EPCR(sEPCR)水平;提取血细胞中的DNA,PCR扩增后将目的片段EPCR基因直接测序,分析EPCR基因第6936位点的多态性.结果 ①正常对照组中,AG基因型组血浆sEPCR水平[(0.97±0.32)ng/L]明显高于AA基因型组[(0.61±0.24)ng/L](P＜0.01);DVT患者组中AG基因型组[(0.87±0.21)ng/L]亦明显高于AA基因型组[(0.50±0.18)ng/L](P＜0.01).②DVT组的血浆sEPCR水平[(0.68±0.32)ng/L]明显高于正常对照组[(0.54±0.22)ng/L](P＜0.05).③EPCR基因6936位点AG基因型分布频率DVT组高于正常对照组(P＜0.05).④AG基因型患DVT的危险性较AA基因型高(OR=2.75,95%可信区间为1.04～7.30)(P＜0.05).结论血浆sEPCR水平与EPCR基因6936A/G多态性有关.DVT患者血浆sEPCR水平较正常人增高.EPCR基因6936 AG基因型者可能患DVT的风险高.
목적 통과병례대조연구,료해내피세포단백C수체(EPCR)기인6936A/G다태성화심정맥혈전형성(DVT)적상관성,진일보료해EPCR재DVT형성중적중요성.방법 용ELISA법검측65례DVT환자화71명건강체검자적외주혈혈장가용성EPCR(sEPCR)수평;제취혈세포중적DNA,PCR확증후장목적 편단EPCR기인직접측서,분석EPCR기인제6936위점적다태성.결과 ①정상대조조중,AG기인형조혈장sEPCR수평[(0.97±0.32)ng/L]명현고우AA기인형조[(0.61±0.24)ng/L](P＜0.01);DVT환자조중AG기인형조[(0.87±0.21)ng/L]역명현고우AA기인형조[(0.50±0.18)ng/L](P＜0.01).②DVT조적혈장sEPCR수평[(0.68±0.32)ng/L]명현고우정상대조조[(0.54±0.22)ng/L](P＜0.05).③EPCR기인6936위점AG기인형분포빈솔DVT조고우정상대조조(P＜0.05).④AG기인형환DVT적위험성교AA기인형고(OR=2.75,95%가신구간위1.04～7.30)(P＜0.05).결론 혈장sEPCR수평여EPCR기인6936A/G다태성유관.DVT환자혈장sEPCR수평교정상인증고.EPCR기인6936 AG기인형자가능환DVT적풍험고.
Objective To investigate the relationship between endothelial protein C receptor(EPCR)gene 6936A/G polymorphism and deep vein thrombosis(DVT). Methods The study group included 65 DVT patients and 71 normal controls. Plasma sEPCR was measured by ELISA. Genomic DNA was extracted by using Genomic Purification Kit. A 315bp EPCR product was amplified by a standard PCR reaction, and the bands were confirmed by direct sequencing after purification. Results ①sEPCR levels in healthy controls with 6936AG genotype were significantly higher than that in those with 6936AA genotype [( 0.97 ± 0. 32 )ng/L vs (0.61 ± 0.24) ng/L, P ＜ 0. 01 )], and so did in DVT patients [(0.87 ± 0. 21 ) ng/L vs (0.50 ±0. 18) ng/L, P ＜ 0. 01]. ②The sEPCR levels of DVT patients [( 0. 68 ± 0. 32) ng/L] were significantly higher than that of healthy controls [(0.54 ±0.22) ng/L] (P＜0.05). ③The distribution of 6936A/G genotype was higher in DVT patients than in healthy controls( P＜0.05 ). ④Subjects with 6936A/G had an increased risk of thrombosis (OR=2.75, 95% CI=1.04-7.30)(P＜0.05). Conclusions EPCR gene 6936A/G polymorphism is associated with increased plasma sEPCR levels. The sEPCR levels in DVT patients were significantly higher than that in healthy controls. The subject with 6936AG likely had an increased risk of thrombosis.