中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2009年
3期
173-176
,共4页
赵世义%张振书%孙嫣%赖桌胜%南清振%李康
趙世義%張振書%孫嫣%賴桌勝%南清振%李康
조세의%장진서%손언%뢰탁성%남청진%리강
结直肠肿瘤%Rho因子%RNA干扰%细胞骨架%肿瘤浸润
結直腸腫瘤%Rho因子%RNA榦擾%細胞骨架%腫瘤浸潤
결직장종류%Rho인자%RNA간우%세포골가%종류침윤
Colorectal neoplasms%Rho factor%RNA interference%Cytoskeleton Neoplasm invasiveness
目的 探讨Racl基因沉默后对结直肠癌LoVo细胞侵袭移动的影响.方法 逆转录PCR和Western印迹法检测Racl mRNA和Racl蛋白在结直肠癌细胞株LoVo、SW480、SW620、SW1116、HT29中的表达.通过转染Racl-shRNA观察Racl基因沉默后LoVo细胞骨架的变化.再转染Rael-N17和Racl-L61,观察Rael基因缺失和表达增强后对LoVo细胞侵袭移动的影响.结果 Rael mRNA和蛋白在结直肠癌细胞株中均高表达.Racl基因沉默后LoVo细胞交联的纤维状肌动蛋白(F-actin)网和细胞膜伪足形成减少.细胞迁移实验显示,Racl-shRNA组细胞迁移数为(75±5)个、Racl-N17组为(93±5)个、Racl-L61组为(267±7)个、对照组为(214±8)个,Racl-shRNA、Racl-N17组与对照组比较差异均有统计学意义(P＜0.01),Racl-L61组与对照组比较差异亦有统计学意义(P＜0.05).在细胞侵袭试验中.Rael-shRNA组细胞迁移数为(35±5)个、Racl-N17为(42±5)个、Racl-L61为(86±7)个、对照组为(73±6)个,Racl-shRNA、Racl-N17组与对照组比较差异均有统计学意义(P＜O.01).Racl-L61组与对照组比较差异亦有统计学意义(P＜0.05).结论 RNA干扰沉默Racl基因后影响了LoVo细胞肌动蛋白细胞骨架的形成.同时抑制了LoVo细胞移动和侵袭特性.
目的 探討Racl基因沉默後對結直腸癌LoVo細胞侵襲移動的影響.方法 逆轉錄PCR和Western印跡法檢測Racl mRNA和Racl蛋白在結直腸癌細胞株LoVo、SW480、SW620、SW1116、HT29中的錶達.通過轉染Racl-shRNA觀察Racl基因沉默後LoVo細胞骨架的變化.再轉染Rael-N17和Racl-L61,觀察Rael基因缺失和錶達增彊後對LoVo細胞侵襲移動的影響.結果 Rael mRNA和蛋白在結直腸癌細胞株中均高錶達.Racl基因沉默後LoVo細胞交聯的纖維狀肌動蛋白(F-actin)網和細胞膜偽足形成減少.細胞遷移實驗顯示,Racl-shRNA組細胞遷移數為(75±5)箇、Racl-N17組為(93±5)箇、Racl-L61組為(267±7)箇、對照組為(214±8)箇,Racl-shRNA、Racl-N17組與對照組比較差異均有統計學意義(P＜0.01),Racl-L61組與對照組比較差異亦有統計學意義(P＜0.05).在細胞侵襲試驗中.Rael-shRNA組細胞遷移數為(35±5)箇、Racl-N17為(42±5)箇、Racl-L61為(86±7)箇、對照組為(73±6)箇,Racl-shRNA、Racl-N17組與對照組比較差異均有統計學意義(P＜O.01).Racl-L61組與對照組比較差異亦有統計學意義(P＜0.05).結論 RNA榦擾沉默Racl基因後影響瞭LoVo細胞肌動蛋白細胞骨架的形成.同時抑製瞭LoVo細胞移動和侵襲特性.
목적 탐토Racl기인침묵후대결직장암LoVo세포침습이동적영향.방법 역전록PCR화Western인적법검측Racl mRNA화Racl단백재결직장암세포주LoVo、SW480、SW620、SW1116、HT29중적표체.통과전염Racl-shRNA관찰Racl기인침묵후LoVo세포골가적변화.재전염Rael-N17화Racl-L61,관찰Rael기인결실화표체증강후대LoVo세포침습이동적영향.결과 Rael mRNA화단백재결직장암세포주중균고표체.Racl기인침묵후LoVo세포교련적섬유상기동단백(F-actin)망화세포막위족형성감소.세포천이실험현시,Racl-shRNA조세포천이수위(75±5)개、Racl-N17조위(93±5)개、Racl-L61조위(267±7)개、대조조위(214±8)개,Racl-shRNA、Racl-N17조여대조조비교차이균유통계학의의(P＜0.01),Racl-L61조여대조조비교차이역유통계학의의(P＜0.05).재세포침습시험중.Rael-shRNA조세포천이수위(35±5)개、Racl-N17위(42±5)개、Racl-L61위(86±7)개、대조조위(73±6)개,Racl-shRNA、Racl-N17조여대조조비교차이균유통계학의의(P＜O.01).Racl-L61조여대조조비교차이역유통계학의의(P＜0.05).결론 RNA간우침묵Racl기인후영향료LoVo세포기동단백세포골가적형성.동시억제료LoVo세포이동화침습특성.
Objective To investigate the effects of silenced Racl on invasion and migration in LOVo cells.Methods The expression of Racl mRNA and protein in colorectal cancer cells(including LoVo SW480.SW620.SW1116,HT29)were detected by RT-PCR and Western blot,respectively.The changes of cytoskeleton were observed in LoVO cells after transfected with Racl-shRNA.then invasion and migration were recorded respectively in LoVo cells after transfected with Racl-N17 and Racl-L61.Results Racl mRNA and protein were overexpressed in all selected colorectal cancer cells.Deletion of Racl decreased the cross-linked actin network and pseudopodia,and inhibited the invasion and migration in LoVo cells.The migration experiment showed that the migrated cells were higher in Racl-shRNA[(75±5)cells].Racl-N17 [(93±5)cells]and Racl-L61[(267±7)cells]groups compared with control group[(214±8)cells,P＜0.01,＜O.01 and＜0.05,resprectively].The invasion experimental study revealed that the migrated cells were higher in Racl-shRNA[(35±5)cells],Racl-N17[(42±5)cells]and Racl-L61[(86±7)cells] groups compared with control group[(73±6)cells,P＜0.01,＜0.01 and＜0.05,resprectively].Condusion Deletoin of Racl can inhibit the invasion and migration in LDVo cells.
