基础医学与临床
基礎醫學與臨床
기출의학여림상
BASIC MEDICAL SCIENCES AND CLINICS
2003年
6期
651-656
,共6页
刘艳梅%张君岚%凌毅群%凌亦凌
劉豔梅%張君嵐%凌毅群%凌亦凌
류염매%장군람%릉의군%릉역릉
核因子kappa B%脂多糖类%中性粒细胞%凋亡基因
覈因子kappa B%脂多糖類%中性粒細胞%凋亡基因
핵인자kappa B%지다당류%중성립세포%조망기인
观测核因子-κB(nuclearfactor kappa B,NF-кB)调控脂多糖(1ipopolysaccharide,LPS)激活的中性粒细胞(polymorphonuclear neutrophil,PMN)凋亡的下游相关基因.将培养的人静脉血PMN分为对照(contro1)组、LPS(100μg/L)组、glotoxin+LPS组及PD098059+LPS组,后两组先用gliotoxin(10mg/L)或PD098059(100μmol/L)培养30min,后加入IPS(100μg/L)继续培养60min.收集细胞,提取总RNA.采用细胞凋亡相关蛋白及应激反应蛋白类基因芯片进行检测.结果显示,control组与LPS组有8条差异基因,3条上调,5条下调;gliotoxin+LPS组与LPS组有10条差异基因,7条上调,3条下调;PD098059+LPS组与LPS组有8条差异基因,5条上调,3条下调.综合分析发现Defender against cell death 1和X-linked inhibitor of apoptosis protein是NF-кB调控PMN凋亡的下游相关基因.Ionizing radiation resistance conferring protein在NF-кB的下游发挥抑制PMN凋亡的作用有待进一步澄清.
觀測覈因子-κB(nuclearfactor kappa B,NF-кB)調控脂多糖(1ipopolysaccharide,LPS)激活的中性粒細胞(polymorphonuclear neutrophil,PMN)凋亡的下遊相關基因.將培養的人靜脈血PMN分為對照(contro1)組、LPS(100μg/L)組、glotoxin+LPS組及PD098059+LPS組,後兩組先用gliotoxin(10mg/L)或PD098059(100μmol/L)培養30min,後加入IPS(100μg/L)繼續培養60min.收集細胞,提取總RNA.採用細胞凋亡相關蛋白及應激反應蛋白類基因芯片進行檢測.結果顯示,control組與LPS組有8條差異基因,3條上調,5條下調;gliotoxin+LPS組與LPS組有10條差異基因,7條上調,3條下調;PD098059+LPS組與LPS組有8條差異基因,5條上調,3條下調.綜閤分析髮現Defender against cell death 1和X-linked inhibitor of apoptosis protein是NF-кB調控PMN凋亡的下遊相關基因.Ionizing radiation resistance conferring protein在NF-кB的下遊髮揮抑製PMN凋亡的作用有待進一步澄清.
관측핵인자-κB(nuclearfactor kappa B,NF-кB)조공지다당(1ipopolysaccharide,LPS)격활적중성립세포(polymorphonuclear neutrophil,PMN)조망적하유상관기인.장배양적인정맥혈PMN분위대조(contro1)조、LPS(100μg/L)조、glotoxin+LPS조급PD098059+LPS조,후량조선용gliotoxin(10mg/L)혹PD098059(100μmol/L)배양30min,후가입IPS(100μg/L)계속배양60min.수집세포,제취총RNA.채용세포조망상관단백급응격반응단백류기인심편진행검측.결과현시,control조여LPS조유8조차이기인,3조상조,5조하조;gliotoxin+LPS조여LPS조유10조차이기인,7조상조,3조하조;PD098059+LPS조여LPS조유8조차이기인,5조상조,3조하조.종합분석발현Defender against cell death 1화X-linked inhibitor of apoptosis protein시NF-кB조공PMN조망적하유상관기인.Ionizing radiation resistance conferring protein재NF-кB적하유발휘억제PMN조망적작용유대진일보징청.
