CAJ | 학술논문

从限氨固氮培养基中培养棕色固氮菌(Azotobacter vinelandii Lipmann)缺失nifE的突变种DJ35中,分离纯化得到缺失FeMoco的钼铁蛋白(ΔnifE Av1).在一定条件下结晶得到深棕色短斜四棱柱晶体.结晶溶液中各组分的浓度以及结晶方法等对其晶核数目、晶体大小和质量有明显影响.目前用气相扩散的悬滴法所得的最大晶体的二维边长分别为0.12 mm和0.13 mm.
종한안고담배양기중배양종색고담균(Azotobacter vinelandii Lipmann)결실nifE적돌변충DJ35중,분리순화득도결실FeMoco적목철단백(ΔnifE Av1).재일정조건하결정득도심종색단사사릉주정체.결정용액중각조분적농도이급결정방법등대기정핵수목、정체대소화질량유명현영향.목전용기상확산적현적법소득적최대정체적이유변장분별위0.12 mm화0.13 mm.
Under a suitable condition of crystallization, dark brown short rhombohedron crystals could be obtained from FeMoco-deficient MoFe protein (ΔnifE Av1) purified from a nifE deleted mutant DJ35 of Azotobacter vinelandii Lipmann grown in NH3-limited medium. The number, size and quality of crystals were significantly affected by either the concentration of precipitants and buffer or diffusion method. The longest sides of the largest crystal of ΔnifE Av1 protein, which was obtained by vapor diffusion in the hanging drop method, were 0.12 and 0.13 mm, respectively.