CAJ | 학술논문

氢化酶是微生物代谢产氢过程中的关键酶,也是目前生物制氢领域的研究热点.本文综述了厌氧发酵产氢微生物中氢化酶的分类及特点,以及[Ni-Fe]、[Fe-Fe]和[Fe-S cluster-free]三种氢化酶晶体结构和活性中心结构;阐述了多种微生物来源的氢化酶结构的研究进展,对几种典型氢化酶的结构及活性中心进行了对比分析,并根据当前研究热点,对氢化酶的研究方向进行了展望.本文阐述的内容信息量丰富且具有一定的实用性,对于氢化酶相关领域研究具有重要的意义.
경화매시미생물대사산경과정중적관건매,야시목전생물제경영역적연구열점.본문종술료염양발효산경미생물중경화매적분류급특점,이급[Ni-Fe]、[Fe-Fe]화[Fe-S cluster-free]삼충경화매정체결구화활성중심결구;천술료다충미생물래원적경화매결구적연구진전,대궤충전형경화매적결구급활성중심진행료대비분석,병근거당전연구열점,대경화매적연구방향진행료전망.본문천술적내용신식량봉부차구유일정적실용성,대우경화매상관영역연구구유중요적의의.
Hydrogenase is the key enzyme and plays an important role in the process of biohydrogen production, which is an interesting topic in this research field. In the paper, the classification of hydrogenase was described. According to lots of research results, the recent reports of crystal structure and active site structure of the three kinds of hydrogenases, [Ni-Fe],[Fe-Fe] and [Fe-S cluster-free], were quoted and contrasted. Moreover, some new approach and direction for hydrogenases were indicated. This review is informative and useful. The overall presentation is good for the relative studies on hydrogenases.