CAJ | 학술논문

目的: 探讨乳化氟碳结合川芎嗪对肝肺综合征(HPS)猪肝移植围手术期肺部炎症的影响及其机制.方法:采用慢性胆管结扎复制猪肝肺综合征模型,随机分为2组:(1)乳化氟碳结合川芎嗪(PFCL)组:肺内灌以20 mL/kg 的全氟碳乳剂(PFC) 及川芎嗪(20 mg/kg,气管内滴注),机械通气下进行原位肝移植;(2)机械通气对照 (MV) 组:常规MV下,进行原位肝移植.实验观察5 h,实验结束后处死动物,取肺脏进行分析.结果:PFCL组肺脏的湿/干比值、肺通透指数及支气管肺泡灌洗液中白细胞计数均显著降低,与MV组比较差异显著(P<0.05);PFCL组肺组织及支气管肺泡灌洗液中肿瘤坏死因子α(TNF-α)、干扰素(IFN)-γ 含量均显著低于MV组(P<0.05).免疫组化染色发现,PFCL组肺组织炎症和上皮细胞核内NF-κB p65的含量明显低于MV组.结论:乳化氟碳结合中药川芎嗪可以有效地减少中性粒细胞浸润,抑制炎性细胞NF-κB的活化和细胞因子TNF-α与IFN-γ的表达,减轻HPS引起的肺损伤.
목적: 탐토유화불탄결합천궁진대간폐종합정(HPS)저간이식위수술기폐부염증적영향급기궤제.방법:채용만성담관결찰복제저간폐종합정모형,수궤분위2조:(1)유화불탄결합천궁진(PFCL)조:폐내관이20 mL/kg 적전불탄유제(PFC) 급천궁진(20 mg/kg,기관내적주),궤계통기하진행원위간이식;(2)궤계통기대조 (MV) 조:상규MV하,진행원위간이식.실험관찰5 h,실험결속후처사동물,취폐장진행분석.결과:PFCL조폐장적습/간비치、폐통투지수급지기관폐포관세액중백세포계수균현저강저,여MV조비교차이현저(P<0.05);PFCL조폐조직급지기관폐포관세액중종류배사인자α(TNF-α)、간우소(IFN)-γ 함량균현저저우MV조(P<0.05).면역조화염색발현,PFCL조폐조직염증화상피세포핵내NF-κB p65적함량명현저우MV조.결론:유화불탄결합중약천궁진가이유효지감소중성립세포침윤,억제염성세포NF-κB적활화화세포인자TNF-α여IFN-γ적표체,감경HPS인기적폐손상.
AIM:To investigate the effects of perfluorocarbon and ligustrazine on lung injury during liver transplantation in pigs with hepatopulmonary syndrome. METHODS:A hepatopulmonary syndrome (HPS) model of pig was established by chronic bile duct ligation. The animals were assigned randomly to 2 groups:(1) Perfluorocarbon in combination with ligustrazine treatment groups (PFCL group):the pigs were treated with intratracheal instillation of perfluorocarbon and ligustrazine; (2) The conventional mechanical ventilation group (MV group):all animals were subjected to mechanical ventilation and orthotopic liver transplantation. After 5 h the lungs were harvested for further analysis. RESULTS:The lung wet to dry weight radio,pulmonary permeation index and leukocyte count in bronchoalveolar lavage fluids (BALF) in PFCL group significantly decreased compared to MV group (P<0.05). Contents of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in the lung tissue,plasma and BALF of pigs in PFCL group were significantly lower than those in MV group (P<0.05). Moreover,the activation of NF-κB was inhibited markedly by PFCL. CONCLUSION:Perfluorocarbon in combination with ligustrazine effectively reduces the PMN accumulation in the lungs,inhibits TNF-α and IFN-γ production and protects against lung injury during liver transplantation in pigs with hepatopulmonary syndrome.