CAJ | 학술논문

在有机溶剂中以维生素E和琥珀酸酐为底物在脂肪酶的催化下合成了维生素E琥珀酸酯.首先对酶促反应的脂肪酶、反应介质和反应温度进行了考察,在所选的几种脂肪酶中,假丝酵母脂肪酶 (Candida sp.) 的催化活性最好;叔丁醇和DMSO组成的混合溶剂(体积比为2∶3)为最合适的反应介质;30℃为适宜的反应温度.并采用Box-Behnken实验设计和响应面因子分析法对底物摩尔比等其他反应条件进行了优化.维生素E琥珀酸酯最优反应条件为:维生素E浓度0.26 mmol·ml~(-1),维生素E和琥珀酸酐摩尔比1∶5,在5 ml叔丁醇和DMSO混合溶剂(体积比为2∶3)中,30℃下在0.02 g Candida sp.脂肪酶的催化下反应71 h,维生素E琥珀酸酯产率达到98.71%.
재유궤용제중이유생소E화호박산항위저물재지방매적최화하합성료유생소E호박산지.수선대매촉반응적지방매、반응개질화반응온도진행료고찰,재소선적궤충지방매중,가사효모지방매 (Candida sp.) 적최화활성최호;숙정순화DMSO조성적혼합용제(체적비위2∶3)위최합괄적반응개질;30℃위괄의적반응온도.병채용Box-Behnken실험설계화향응면인자분석법대저물마이비등기타반응조건진행료우화.유생소E호박산지최우반응조건위:유생소E농도0.26 mmol·ml~(-1),유생소E화호박산항마이비1∶5,재5 ml숙정순화DMSO혼합용제(체적비위2∶3)중,30℃하재0.02 g Candida sp.지방매적최화하반응71 h,유생소E호박산지산솔체도98.71%.
Vitamin E succinate was synthesized via acylation of vitamin E with succinic anhydride using Candida sp.lipase as catalyst.The effects of enzyme sources,organic media and reaction temperature on the reaction yield were investigated.The results showed that Candida sp.lipase was the best lipase,the mixture of tert-butanol and DMSO (2∶3,vol) was the most suitable media for the reaction,and the appropriate reaction temperature was 30℃.Response surface methodology (RSM) and three-level-three-factor Box-Behnken design were used to evaluate the effects of other synthesis parameters,such as reaction time,substrates concentration and the molar ratio of vitamin E to succinic anhydride on the yield of vitamin E succinate.By solving the quadratic regression model equation using appropriate statistic methods,the optimum synthesis conditions were as follows:reaction time 71 h,vitamin E concentration 0.26 mmol·ml~(-1),vitamin E to succinic anhydride molar ratio 1∶5.The predicted value of the vitamin E succinate yield was 99.27%.The actual experimental value 98.71% was in good agreement of the predicted value.