CAJ | 학술논문

目的探讨血管束植入组织工程骨修复兔股骨缺损对局部血管内皮生长因了(VEGF)表达的影响.方法将兔自体骨髓基质下细胞经诱导后与β-磷酸三钙材料复合,植入制备的兔股骨缺损处,其中实验组在材料侧槽中植入股骨的动静脉血管束,对照组则单纯植入组织工稗骨,分别于术后2、4、8、12周通过形态学检测新生骨量,免疫组织化学方法检测新生骨中VEGF的表达量.结果随着时间进展,各组成骨量逐渐增加,差异有统计学意义(P<0.05),并且从第4周开始实验组成骨量高于对照组,差异有统计学意义(P<0.05);符时问点实验组新生骨中VEGF的表达最均高于对照组,差异有统汁学意义(P<0.05),且4周时达到最人值.结论血管束植入组织工程骨可明显增加新生骨中VEGF的表达并能促进骨缺损的修复.
목적 탐토혈관속식입조직공정골수복토고골결손대국부혈관내피생장인료(VEGF)표체적영향.방법 장토자체골수기질하세포경유도후여β-린산삼개재료복합,식입제비적토고골결손처,기중실험조재재료측조중식입고골적동정맥혈관속,대조조칙단순식입조직공패골,분별우술후2、4、8、12주통과형태학검측신생골량,면역조직화학방법검측신생골중VEGF적표체량.결과 수착시간진전,각조성골량축점증가,차이유통계학의의(P<0.05),병차종제4주개시실험조성골량고우대조조,차이유통계학의의(P<0.05);부시문점실험조신생골중VEGF적표체최균고우대조조,차이유통즙학의의(P<0.05),차4주시체도최인치.결론 혈관속식입조직공정골가명현증가신생골중VEGF적표체병능촉진골결손적수복.
Objective To investigate whether tissue engineered bone with implanted vascular bun-dles can up-regulate release of vascular endothelial growth factor (VEGF) in models of femoral defect in rabbits.Methods Thirty-two rabbits were randomized into 2 even groups.In both groups, a segmental bone defect of 15 mm in length was made at the left femur before a tissue engineered bone was inserted into the defect.In the experimental group, a femoral vascular bundle was implanted into the tissue engineered bone.In the control group, there was no vascular implantation.At 2, 4, 8, and 12 weeks after implantation, samples were taken to determine new bone formation by histology and expression level of VEGF by immuno-histochemistry.Results The new bone formation was significantly higher in the experimental group at the end of 4, 8, and 12 weeks(P < 0.05) .The expression level of VEGF in the experimental group was also significantly higher than in the control group at all time points after operation, and the expression of VEGF peaked at 4 weeks.Conclusion Tissue engineered bone with vascular bundle implanted can up-regulate VEGF release in models of femoral defect in rabbits.