CAJ | 학술논문

目的探讨人脐带血间充质干细胞(hUCB MSCs)在体分化为皮肤细胞、修复皮肤缺损创面的可能性. 方法分离hUCB MSCs,体外培养、扩增并用BrdU标记后,种植于重症联合免疫缺陷(SCID)小鼠背部全层皮肤缺损创面(治疗组),并设同体PBS对照组.于术后7,14 d观察并比较愈合率;于术后7,14,28 d创面活检,行常规病理组织切片HE染色、免疫组织化学染色检测BrdU及人角蛋白19的表达. 结果术后7,14 d治疗组创面愈合率为(56.06±3.04)%和(94.75±1.89)%,对照组为(36.99 ±2.17)%和(84.63 ±2.28)%,治疗组高于对照组(P＜0.01).HE染色显示治疗组活检组织表皮层明显增厚,细胞及表皮嵴数目显著增多;在再生表皮的毛囊内层以及表皮基底层、棘层可见BrdU阳性标记细胞连续分布,且有部分细胞表达人角蛋白19;并在治疗组活检组织中发现有皮肤附属器形成. 结论 hUCB MSCs可在体分化为皮肤细胞与组织,可应用于皮肤创面的修复.
목적 탐토인제대혈간충질간세포(hUCB MSCs)재체분화위피부세포、수복피부결손창면적가능성. 방법 분리hUCB MSCs,체외배양、확증병용BrdU표기후,충식우중증연합면역결함(SCID)소서배부전층피부결손창면(치료조),병설동체PBS대조조.우술후7,14 d관찰병비교유합솔;우술후7,14,28 d창면활검,행상규병리조직절편HE염색、면역조직화학염색검측BrdU급인각단백19적표체. 결과 술후7,14 d치료조창면유합솔위(56.06±3.04)%화(94.75±1.89)%,대조조위(36.99 ±2.17)%화(84.63 ±2.28)%,치료조고우대조조(P＜0.01).HE염색현시치료조활검조직표피층명현증후,세포급표피척수목현저증다;재재생표피적모낭내층이급표피기저층、극층가견BrdU양성표기세포련속분포,차유부분세포표체인각단백19;병재치료조활검조직중발현유피부부속기형성. 결론 hUCB MSCs가재체분화위피부세포여조직,가응용우피부창면적수복.
Objective To study the probability of applying mesenchymal stem cells isolated from human umbilical cord blood (hUCB MSCs) to repair mouse skin wound in vivo. Methods hUCB MSCs isolated from full term delivery human umbilical cord blood were cultured and amplified in vitro.hUCB MSCs at passage 9 were labeled with BrdU (5-bromodeoxy-uridine) and grafted on the full-thickness skin loss wound created on the back of the severe combined immunodeficiency (SCID) mouse (treatment group), when a PBS control group was set. The wound healing rate was surveyed and compared at days 7 and 14 postoperatively. Meanwhile, the wound was biopsied at days 7, 14 and 28 after operation,and the expressions of BrdU antibody and K19 antibody were checked pathologically and immunohistochemically by HE staining, respectively. Results The wound in treatment group was healed more rapidly than that in control group (P ＜ 0.01 ). The pathological check of the biopsy sample showed that the epidermis was thicker, with more epidermal ridges in the treatment group, compared with control group.It was found that some BrdU positive cells were distributed successively on the hair follicle, the stratum basal and the spinosum layers, a few of which even expressed K19. Conclusion hUCB MSCs can be differentiated into skin tissue and cells and is possible to repair skin wound.