中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2010年
11期
1008-1011
,共4页
张书平%祝家群%黄汉津%郑园园
張書平%祝傢群%黃漢津%鄭園園
장서평%축가군%황한진%정완완
帕金森病%1-甲基-4-苯基-1,2,3,6-四氢吡啶%多巴胺%小鼠%学习记忆
帕金森病%1-甲基-4-苯基-1,2,3,6-四氫吡啶%多巴胺%小鼠%學習記憶
파금삼병%1-갑기-4-분기-1,2,3,6-사경필정%다파알%소서%학습기억
Parkinson's disease%1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)%Dopamine%Mice%Learning and memory
目的 比较1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)所致的急性、亚急性和慢性帕金森病(PD)模型小鼠空间参考记忆和脑内多巴胺(DA)水平的差异.方法 用相同的MPTP使用总量不同的注射间隔分别制作急性、亚急性和慢性PD小鼠模型.Morris水迷宫检测小鼠空间参考记忆,高效液相(HPLC)检测纹状体、海马和前额叶皮层的DA含量,免疫组化染色检测黑质酪氨酸羟化酶(TH)阳性神经元数量.结果 慢性PD小鼠的逃避潜伏期较对照组有明显延长(P＜0.05),而急性和亚急性PD小鼠则无明显变化.3组实验组纹状体DA含量[(1180.1±293.0)ng/ml,(1177.4±450.5)ng/ml,(1149.6±353.0)ng/ml]均较对照组[(225.6±79.7)ng/ml,(273.6±64.9)ng/ml,(327.1±126.2)ng/ml]有显著减少(P＜0.01),急性PD小鼠前额叶皮层DA含量[(65.3±23.9)ng/ml]较对照组[(41.2±18.8)ng/ml]有显著减少(P＜0.05),3组实验组海马DA含量无明显变化.3组实验组黑质TH阳性神经元数量均较对照组有显著减少(P＜0.05).结论 3种PD模型小鼠空间参考记忆差异可能不是由其脑内DA水平差异所致.
目的 比較1-甲基-4-苯基-1,2,3,6-四氫吡啶(MPTP)所緻的急性、亞急性和慢性帕金森病(PD)模型小鼠空間參攷記憶和腦內多巴胺(DA)水平的差異.方法 用相同的MPTP使用總量不同的註射間隔分彆製作急性、亞急性和慢性PD小鼠模型.Morris水迷宮檢測小鼠空間參攷記憶,高效液相(HPLC)檢測紋狀體、海馬和前額葉皮層的DA含量,免疫組化染色檢測黑質酪氨痠羥化酶(TH)暘性神經元數量.結果 慢性PD小鼠的逃避潛伏期較對照組有明顯延長(P＜0.05),而急性和亞急性PD小鼠則無明顯變化.3組實驗組紋狀體DA含量[(1180.1±293.0)ng/ml,(1177.4±450.5)ng/ml,(1149.6±353.0)ng/ml]均較對照組[(225.6±79.7)ng/ml,(273.6±64.9)ng/ml,(327.1±126.2)ng/ml]有顯著減少(P＜0.01),急性PD小鼠前額葉皮層DA含量[(65.3±23.9)ng/ml]較對照組[(41.2±18.8)ng/ml]有顯著減少(P＜0.05),3組實驗組海馬DA含量無明顯變化.3組實驗組黑質TH暘性神經元數量均較對照組有顯著減少(P＜0.05).結論 3種PD模型小鼠空間參攷記憶差異可能不是由其腦內DA水平差異所緻.
목적 비교1-갑기-4-분기-1,2,3,6-사경필정(MPTP)소치적급성、아급성화만성파금삼병(PD)모형소서공간삼고기억화뇌내다파알(DA)수평적차이.방법 용상동적MPTP사용총량불동적주사간격분별제작급성、아급성화만성PD소서모형.Morris수미궁검측소서공간삼고기억,고효액상(HPLC)검측문상체、해마화전액협피층적DA함량,면역조화염색검측흑질락안산간화매(TH)양성신경원수량.결과 만성PD소서적도피잠복기교대조조유명현연장(P＜0.05),이급성화아급성PD소서칙무명현변화.3조실험조문상체DA함량[(1180.1±293.0)ng/ml,(1177.4±450.5)ng/ml,(1149.6±353.0)ng/ml]균교대조조[(225.6±79.7)ng/ml,(273.6±64.9)ng/ml,(327.1±126.2)ng/ml]유현저감소(P＜0.01),급성PD소서전액협피층DA함량[(65.3±23.9)ng/ml]교대조조[(41.2±18.8)ng/ml]유현저감소(P＜0.05),3조실험조해마DA함량무명현변화.3조실험조흑질TH양성신경원수량균교대조조유현저감소(P＜0.05).결론 3충PD모형소서공간삼고기억차이가능불시유기뇌내DA수평차이소치.
Objective To compare the difference of spatial reference memory and dopamine (DA) level in the brain between acute,subacute and chronic mouse model of Parkinson' s disease(PD) induced by 1-methyl-4-phenyl-1 ,2,3,6-tetrahydropyridine(MPTP) injection. Methods The acute,subacute and chronic mouse model of Parkinson' s disease were induced by injecting the same MPTP volume dose with different schedules. The spatial reference memory of mice was tested by morris water maze. Dopamine concentration in striatum, hippocampus and the prefrontal cortex were detected with HPLC. The number of tyrosine hydroxylase(TH)-positive cells in the substantia nigra of mice was detected by immunohistochemistry. Results The mean escape latency of the chronic but not the acute or subacute mouse model of Parkinson' s disease was significant longer ( P ＜ 0.05 ) than its control group. The striatum DA concentration of three test groups ( ( 1180. 1 ± 293.0 ) ng/ml, ( 1177.4 ± 450.5 ) ng/ml,( 1149.6 ± 353.0 ) ng/ml ) reduced significantly compared to their control groups ( ( 225.6 ± 79.7 ) ng/ml, ( 273.6± 64.9 ) ng/ml, ( 327. 1 ± 126.2 ) ng/ml, P ＜ 0.01 ). The prefrontal cortex DA concentration of the acute mouse model of Parkinson' s disease ( ( 65.3 ± 23.9 ) ng/ml ) was significant lower than its control group ( ( 41.2 ±18.8 )ng/ml, P ＜ 0.05 ). No significant changes of hippocampus DA concentration were seen between these test groups and their control groups. The number of TH positive cells in substantia nigra significantly decreased in three test groups compared to their control groups( P ＜ 0.05 ). Conclusion The difference of spatial reference memory between three regimens of the mouse model of Parkinson' s disease may not due to the difference of DA level in their brain.
