国际脑血管病杂志
國際腦血管病雜誌
국제뇌혈관병잡지
INTERNATIONAL JOURNAL OF CEREBROVASCULAR DISEASES
2012年
2期
142-146
,共5页
朱震寒%沙杜鹃%李启明%李瑾%韩勇%顾双双%张均
硃震寒%沙杜鵑%李啟明%李瑾%韓勇%顧雙雙%張均
주진한%사두견%리계명%리근%한용%고쌍쌍%장균
可卡因-苯丙胺调节转录蛋白%脑缺血%卒中%4-羟壬烯醛%神经保护药%小鼠
可卡因-苯丙胺調節轉錄蛋白%腦缺血%卒中%4-羥壬烯醛%神經保護藥%小鼠
가잡인-분병알조절전록단백%뇌결혈%졸중%4-간임희철%신경보호약%소서
Cocaine- And Amphetamine-Regulated Transcript Protein%Brain Ischemia%Stroke%4-Hydroxy-2-Nonenal%Neuroprotective Agents%Mice
目的 探讨可卡因-苯丙胺调节转录肽(cocaine- and amphetamine-regulated transcript peptides,CART)对缺血再灌注小鼠梗死体积和脑组织4-羟壬烯醛(4-hydroxy-2 -noneral,HNE)含量的影响.方法 健康雄性小鼠随机分为缺血再灌注组(n=27)、CART组(n=27)、生理盐水对照组(n=27)和假手术组(n=15).建立大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)模型,CART组和生理盐水对照组在MCAO2 h后分别经尾静脉注射给予CART55-102和同体积生理盐水,然后每隔24 h重复1次.在再灌注后12、24、48和72 h分别进行神经功能评分、梗死体积和脂质氧化应激代谢产物HNE含量检测.结果 CART可显著改善脑缺血再灌注后不同时间点神经功能缺损评分(P均＜0.05),显著缩小梗死体积(P均＜0.05)；再灌注后各时间点HNE含量均显著增高(P均＜0.05),CART可显著下调缺血后脑内HNE水平升高(P均＜0.05).结论 CART能通过抑制脂质过氧化保护小鼠缺血性脑损伤.
目的 探討可卡因-苯丙胺調節轉錄肽(cocaine- and amphetamine-regulated transcript peptides,CART)對缺血再灌註小鼠梗死體積和腦組織4-羥壬烯醛(4-hydroxy-2 -noneral,HNE)含量的影響.方法 健康雄性小鼠隨機分為缺血再灌註組(n=27)、CART組(n=27)、生理鹽水對照組(n=27)和假手術組(n=15).建立大腦中動脈閉塞(middle cerebral artery occlusion,MCAO)模型,CART組和生理鹽水對照組在MCAO2 h後分彆經尾靜脈註射給予CART55-102和同體積生理鹽水,然後每隔24 h重複1次.在再灌註後12、24、48和72 h分彆進行神經功能評分、梗死體積和脂質氧化應激代謝產物HNE含量檢測.結果 CART可顯著改善腦缺血再灌註後不同時間點神經功能缺損評分(P均＜0.05),顯著縮小梗死體積(P均＜0.05)；再灌註後各時間點HNE含量均顯著增高(P均＜0.05),CART可顯著下調缺血後腦內HNE水平升高(P均＜0.05).結論 CART能通過抑製脂質過氧化保護小鼠缺血性腦損傷.
목적 탐토가잡인-분병알조절전록태(cocaine- and amphetamine-regulated transcript peptides,CART)대결혈재관주소서경사체적화뇌조직4-간임희철(4-hydroxy-2 -noneral,HNE)함량적영향.방법 건강웅성소서수궤분위결혈재관주조(n=27)、CART조(n=27)、생리염수대조조(n=27)화가수술조(n=15).건립대뇌중동맥폐새(middle cerebral artery occlusion,MCAO)모형,CART조화생리염수대조조재MCAO2 h후분별경미정맥주사급여CART55-102화동체적생리염수,연후매격24 h중복1차.재재관주후12、24、48화72 h분별진행신경공능평분、경사체적화지질양화응격대사산물HNE함량검측.결과 CART가현저개선뇌결혈재관주후불동시간점신경공능결손평분(P균＜0.05),현저축소경사체적(P균＜0.05)；재관주후각시간점HNE함량균현저증고(P균＜0.05),CART가현저하조결혈후뇌내HNE수평승고(P균＜0.05).결론 CART능통과억제지질과양화보호소서결혈성뇌손상.
Objective To investigate the effect of cocaine-amphetamine-regulated transcript peptide (CART) on the content of 4-hydroxy-2-noneral (HNE) and infarct volume after cerebral ischemia/reperfusion in mice.Methods A total of 96 healthy male mice were randomly divided into four groups:ischemia/reperfusion (n =27),CART (n =27),normal saline control (n =27) and sham operation (n =15) groups.A middle cerebral artery occlusion (MCAO) model was induced.Two hours after MCAO,CART 55-102 and equivalent normal saline were injected respectively via the tail veins of mice in the CART group and the normal saline control group,and then they were injected every other 24 hour.The neurological scores,infarct volume and the HNE content of lipid metabolism of oxidative stress were performed and detected respectively at 12,24,48 and 72hours after reperfusion.Results CART could significantly improve the neurological deficit scores (all P ＜0.05) and reduce infarct volume (all P＜0.05) at different time points after ischemia/reperfusion.The content of HNE was upregulated (all P＜0.05) at different points after referfusion.CART could significantly down-regulate the increased HNE levd in brain after ischemia (all P＜0.05).Conclusions CART may protect ischemic brain injury in mice by inhibiting lipid peroxidation.
