CAJ | 학술논문

目的观察脂联素对肝星状细胞(HSCs)分泌结缔组织生长因子(CTGF)的影响,并观察Ⅲ型前胶原(PCⅢ)、透明质酸(HA)含量的变化.方法把大鼠HSCs分成5组,其中4组分别加入不同浓度的脂联素,另外1组作对照.通过RT-PCR和Western Blot技术分析CTGF mRNA和蛋白表达水平,ELISA法检测上清液PCⅢ、HA含量.结果与对照组比较,A、B、C、D组脂联素HSCs内CTGF mRNA表达均下降,吸光度比值分别为1.54±0.18、1.21±0.14、0.96±0.10、0.79±0.08,其中以D组下降最明显(t=2.42,P＜0.05;t=2.73,P＜0.05;t=3.28,P＜0.01;t=4.67,P＜0.01);CTGF蛋白表达也抑制,积分吸光度比值分别为1.54±0.18、1.21±0.14、0.96±0.10、0.79±0.08,其中以D组抑制最明显(t=2.84,P＜0.01;t=4.05,P＜0.01;t=6.25,P＜0.01;t=9.72,P＜0.01);上清液PCⅢ、HA含量也下降,随脂联素浓度增加,下降越明显.结论脂联素能够抑制HSCs分泌PCⅢ、HA;脂联素可通过抑制HSCs内CTGF表达而发挥抗纤维化作用.
목적 관찰지련소대간성상세포(HSCs)분비결체조직생장인자(CTGF)적영향,병관찰Ⅲ형전효원(PCⅢ)、투명질산(HA)함량적변화.방법 파대서HSCs분성5조,기중4조분별가입불동농도적지련소,령외1조작대조.통과RT-PCR화Western Blot기술분석CTGF mRNA화단백표체수평,ELISA법검측상청액PCⅢ、HA함량.결과 여대조조비교,A、B、C、D조지련소HSCs내CTGF mRNA표체균하강,흡광도비치분별위1.54±0.18、1.21±0.14、0.96±0.10、0.79±0.08,기중이D조하강최명현(t=2.42,P＜0.05;t=2.73,P＜0.05;t=3.28,P＜0.01;t=4.67,P＜0.01);CTGF단백표체야억제,적분흡광도비치분별위1.54±0.18、1.21±0.14、0.96±0.10、0.79±0.08,기중이D조억제최명현(t=2.84,P＜0.01;t=4.05,P＜0.01;t=6.25,P＜0.01;t=9.72,P＜0.01);상청액PCⅢ、HA함량야하강,수지련소농도증가,하강월명현.결론 지련소능구억제HSCs분비PCⅢ、HA;지련소가통과억제HSCs내CTGF표체이발휘항섬유화작용.
Objective To observe the effects of adiponectin on mRNA and protein expressions of connective tissue growth factor (CTGF) in hepatic stellate cells (HSCs) and the levels of procollagen type Ⅲ (PC Ⅲ) and hyaluronic acid (HA).Methods Cultured rat HSCs were treated with different concentrations of adiponectin.CTGF mRNA and protein expressions were analyzed by reverse transcription polymerase chain reaction (RT-PCR) and Western Blot.The levels of PCⅢ and HA were detected by enzymelinked immunosorbent assay (ELISA).Results Compared with the control group,the result of RT-PCR showed that the four groups had different degrees of inhibitory effect,of which group D exhibited the strongest inhibitory effect.The absorbance ratio was 1.54 ±0.18,1.21 ±0.14,0.96 ±0.10,and 0.79 ± 0.08,respectively (t =2.42,P ＜0.05;t =2.73,P ＜0.05;t =3.28,P ＜0.01;t =4.67,P ＜0.01).Western Blot also indicated that four groups had different degrees of inhibitory effect,of which D group exhibited the strongest inhibitory effect.The ratio of integral absorption was 1.54 ± 0.18,1.21 ±0.14,0.96±0.10,and 0.79 ±0.08,respectively (t =2.84,P ＜0.01;t =4.05,P ＜0.01;t =6.25,P ＜0.01;t =9.72,P ＜0.01).The levels of PCⅢ and HA secreted in culture media were also decreased.It was significantly decreased with the concentration of adiponectin increased.Conclusion Adiponectin can inhibit the levels of PC Ⅲ and HA,which may be achieved through reducing CTGF mRNA and protein expressions.