CAJ | 학술논문

目的探讨应用细胞DNA定量分析方法鉴别诊断良、恶性乳腺包块的价值,为临床制定手术方法和预后方案提供依据.方法 488 例乳腺包块住院患者被纳入研究对象,诊断医师进行针吸穿刺,然后将样品制成2张薄层涂片.1张涂片做巴氏染色,用于常规细胞学检查,另一张经 Feulgen染色,用自动细胞DNA定量分析系统检测.每例均进行术后病理观察.结果 488例乳腺肿物经病理证实,164例为乳腺良性肿瘤,324例为恶性肿瘤.常规细胞学方法对乳腺肿瘤的灵敏度为91.4%(296/324)、特异性为92.7%(152/164);而细胞DNA定量分析方法的灵敏度为90.1%(292/324)、特异性为100.0%(164/164).常规细胞学方法阳性预测值为96.1%(296/308),阴性预测值为84.4%(152/180),而细胞DNA定量分析方法中阳性预测值为100.0%(292/292),阴性预测值为83.7%(164/196).结论细胞DNA定量分析可用于乳腺肿块术前诊断中恶性程度的判断.
목적 탐토응용세포DNA정량분석방법감별진단량、악성유선포괴적개치,위림상제정수술방법화예후방안제공의거.방법 488 례유선포괴주원환자피납입연구대상,진단의사진행침흡천자,연후장양품제성2장박층도편.1장도편주파씨염색,용우상규세포학검사,령일장경 Feulgen염색,용자동세포DNA정량분석계통검측.매례균진행술후병리관찰.결과 488례유선종물경병리증실,164례위유선량성종류,324례위악성종류.상규세포학방법대유선종류적령민도위91.4%(296/324)、특이성위92.7%(152/164);이세포DNA정량분석방법적령민도위90.1%(292/324)、특이성위100.0%(164/164).상규세포학방법양성예측치위96.1%(296/308),음성예측치위84.4%(152/180),이세포DNA정량분석방법중양성예측치위100.0%(292/292),음성예측치위83.7%(164/196).결론 세포DNA정량분석가용우유선종괴술전진단중악성정도적판단.
Objective To explore the application of quantitative DNA analysis in differential diagnosis of benign and malignant breast masses to aid the surgery plan formation.Methods Four hundred and eighty-eight patients with breast mass were enrolled into this study.Tissues of breast mass in patients were gained by fine-needle aspiration puncture.Two sections were made from each sample,one was stained by Papanicolaou for regular cytology analysis and another was stained with Feulgen for quantitative DNA analysis.Pathological results were confirmed in each case after surgery.Results One hundred and sixty-four cases were classified as patients with benign neoplasm,while the other 324 cases were classified as malignant neoplasm,according to the pathological examination results.The sensibility and specificity were 91.4%(296/324) and 92.7%(152/164) for regular cytological method,90.1%(292/324) and 100.0%(164/164) for quantitative DNA analysis method.Meanwhile the positive predictive and negtive value of quantitative DNA analysis was 100.0%(292/292) and 83.7%(164/164),of which regular cytological methods were 96.1%(296/308) and 84.4%(152/180).Conclusion The quantitative DNA analysis might assistant differential diagnosis of benign and malignant breast tumor.