CAJ | 학술논문

目的探讨新疆地区紫色毛癣菌的基因特点,为新疆儿童头癣致病菌的分子流行病学研究提供依据.方法用5种限制性内切酶HaeⅢ,BglⅠ,MspⅠ,DdeⅠ及MboⅠ,采用PCR限制性片段长度多态性(RFLP)法对分离于新疆儿童头癣的30株紫色毛癣菌的rDNA非转录间隔(NTS)区进行基因分型,并与北京的9株、台湾的2株紫色毛癣菌做比较.结果内切酶DdeⅠ将全部41株菌分为12个基因型.新疆的30株菌分为10个基因型,其中17株菌分为D、F、G、H、I、J、K 7个基因型,与北京和台湾菌株间有明显基因差异,其余13株菌分3个基因型,与北京和台湾菌株有基因同源性.结论新疆地区儿童头癣分离的紫色毛癣菌既有独特基因型,又与北京、台湾菌株有基因同源性,体现了新疆紫色毛癣菌的基因多态性.
목적 탐토신강지구자색모선균적기인특점,위신강인동두선치병균적분자류행병학연구제공의거.방법 용5충한제성내절매HaeⅢ,BglⅠ,MspⅠ,DdeⅠ급MboⅠ,채용PCR한제성편단장도다태성(RFLP)법대분리우신강인동두선적30주자색모선균적rDNA비전록간격(NTS)구진행기인분형,병여북경적9주、태만적2주자색모선균주비교.결과 내절매DdeⅠ장전부41주균분위12개기인형.신강적30주균분위10개기인형,기중17주균분위D、F、G、H、I、J、K 7개기인형,여북경화태만균주간유명현기인차이,기여13주균분3개기인형,여북경화태만균주유기인동원성.결론 신강지구인동두선분리적자색모선균기유독특기인형,우여북경、태만균주유기인동원성,체현료신강자색모선균적기인다태성.
Objective To analyze the genotypic characteristics of T. violaceum, and offer evidence for molecular epidemiological study of causative agents of pediatric tinea capitis, in Xinjiang Uygur Autonomous Region. Methods The PCR-restriction fragment length polymorphisim (RFLP) of the nontranscribed spacer region of ribosomal DNA (rDNA-NTS) was assessed by using 5 restriction enzymes, including HaeⅢ, Bgl Ⅰ,MspⅠ, DdeⅠ and MboⅠ, for 30 clinical isolates of T. violaceum from children with tinea capitis in Xinjiang Uygur Autonomous Region. Nine strains of T. violaceum from Beijing and 2 strains from Taiwan served as the control.Results All the 41 strains of T. violaceum were classified into 12 genotypes with the restriction enzyme Ddel.Ten genotypes were revealed in the 30 strains from Xinjiang; among them, 17 strains showed 7 different genotypes (D, F, G, H, I, J and K) with a high intraspecies diversity in comparison with the Beijing and Taiwan isolates; the remaining 13 strains from Xinjiang showed 3 genotypes, which were shared by the Beijing and Taiwan isolates. Conclusions The T. violaceum isolates from children with tinea capitis in Xinjiang Uygur Autonomous Region have unique genotypes, but also show genetic homogeneity with the strains from Beijing and Taiwan, hinting the genetic diversity of T. violaceum.