中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2012年
5期
481-485
,共5页
曾令成%王煜%万锋%叶飞%郭东生%雷霆
曾令成%王煜%萬鋒%葉飛%郭東生%雷霆
증령성%왕욱%만봉%협비%곽동생%뢰정
神经胶质瘤%肿瘤干细胞%抗原,CD133%剪接变体
神經膠質瘤%腫瘤榦細胞%抗原,CD133%剪接變體
신경효질류%종류간세포%항원,CD133%전접변체
Glioma%Neoplastic stem cell%Antigens,CD133%Splice variant
目的 判断CD133剪接变体的表达与AC133抗原决定簇的关系.方法 对AC133阳性和阴性胶质瘤细胞中剪接变体的表达进行了对比,并进行了AC133阳性胶质瘤干细胞诱导分化下CD133剪接变体的表达研究.以RT - PCR针对性的扩充CD133 mRNA条件外显子集中序列,并通过DNA测序确定其条件外显子,同时以实时荧光定量PCR进行了条件外显子表达的定量分析.结果 AC133阳性和阴性细胞均表达相同的CD133 mRNA剪接变体,剪接变体的表达与CD133蛋白的亚细胞分布无关联.AC133阳性胶质瘤干细胞诱导分化过程中,含3号条件外显子的剪接变体的表达显著上调(P＜0.05).结论 在脑胶质瘤中,未能证实与AC133抗原决定簇表达呈特异关联的剪接变体,含3号条件外显子的剪接变体表达与胶质瘤细胞的分化表型相关联.
目的 判斷CD133剪接變體的錶達與AC133抗原決定簇的關繫.方法 對AC133暘性和陰性膠質瘤細胞中剪接變體的錶達進行瞭對比,併進行瞭AC133暘性膠質瘤榦細胞誘導分化下CD133剪接變體的錶達研究.以RT - PCR針對性的擴充CD133 mRNA條件外顯子集中序列,併通過DNA測序確定其條件外顯子,同時以實時熒光定量PCR進行瞭條件外顯子錶達的定量分析.結果 AC133暘性和陰性細胞均錶達相同的CD133 mRNA剪接變體,剪接變體的錶達與CD133蛋白的亞細胞分佈無關聯.AC133暘性膠質瘤榦細胞誘導分化過程中,含3號條件外顯子的剪接變體的錶達顯著上調(P＜0.05).結論 在腦膠質瘤中,未能證實與AC133抗原決定簇錶達呈特異關聯的剪接變體,含3號條件外顯子的剪接變體錶達與膠質瘤細胞的分化錶型相關聯.
목적 판단CD133전접변체적표체여AC133항원결정족적관계.방법 대AC133양성화음성효질류세포중전접변체적표체진행료대비,병진행료AC133양성효질류간세포유도분화하CD133전접변체적표체연구.이RT - PCR침대성적확충CD133 mRNA조건외현자집중서렬,병통과DNA측서학정기조건외현자,동시이실시형광정량PCR진행료조건외현자표체적정량분석.결과 AC133양성화음성세포균표체상동적CD133 mRNA전접변체,전접변체적표체여CD133단백적아세포분포무관련.AC133양성효질류간세포유도분화과정중,함3호조건외현자적전접변체적표체현저상조(P＜0.05).결론 재뇌효질류중,미능증실여AC133항원결정족표체정특이관련적전접변체,함3호조건외현자적전접변체표체여효질류세포적분화표형상관련.
Objective To determine the relationship between CD133 mRNA splice variant and AC133 epitope.Methods The expression of CD133 mRNA splice variants was compared between AC133 positive and negative glioma cells under different culture conditions or by FACS sort.The splice variants expression in AC133 positive glioms stem cells under differentiation was also studied. RT - PCR was performed to specifically amplify the CD133 mRNA sequence containing facultative exons,which were further sequenced to determine the expressed facultative exons. Meanwhile,the real - time quantitative fluorescent PCR was performed to quantify the facultative exons.Results AC133 positive and negative glioma cells from different culture condition or by FACS sort expressed the same splice variants. No correlation between splice variants expression and CD133 protein sub - cellular location was found.The relative expression of splice variants containing facultative exon3 was up - regulated in AC133 positive glioma stem cells under differentiation( P ＜ 0.05 ).Conclusions In glioma no specific CD133 mRNA splice variant for AC133 epitope is identified. Splice variants containing facultative exon3 correlate with the differentiated phenotype of glioma cells.
