中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
1999年
1期
48-49
,共2页
环孢素类%喉%移植%活组织检查
環孢素類%喉%移植%活組織檢查
배포소류%후%이식%활조직검사
Cyclosporins%Larynx%Transplantation%Biopsy
目的 研究环孢素A(CsA)对同种异体喉移植物排斥反应的抑制功效、相应的组织学改变以及与CsA应用剂量之间的关系.方法 供体为封闭群SD大鼠,受体为Wistar大鼠,将受体分为对照组和两个实验组,实验Ⅰ组和Ⅱ组每天分别经腹腔注射CsA 15 mg·kg-1·d-1和25mg·kg-1·d-1,连续2周.通过受体局部解剖所见和组织学检查来判定同种异体移植物是否存活.结果 对照组移植物均出现了明显的急性排斥反应.两实验组术后7天组织学变化较对照组明显为轻,14天时喉移植物的组织结构保持完整.实验Ⅱ组14天时有2只动物组织检查显示粘膜及粘膜下层充血水肿和以多形核白细胞为主的炎性细胞浸润,粘膜上皮坏死脱落,溃疡形成和腺体坏死消失等感染征象,另1只则仅表现为轻度的排斥反应.结论 CsA能有效阻止受体急性排斥反应的发生,其功效与给药途径和剂量关系密切,大剂量CsA的应用可能会增加受体的感染机会.
目的 研究環孢素A(CsA)對同種異體喉移植物排斥反應的抑製功效、相應的組織學改變以及與CsA應用劑量之間的關繫.方法 供體為封閉群SD大鼠,受體為Wistar大鼠,將受體分為對照組和兩箇實驗組,實驗Ⅰ組和Ⅱ組每天分彆經腹腔註射CsA 15 mg·kg-1·d-1和25mg·kg-1·d-1,連續2週.通過受體跼部解剖所見和組織學檢查來判定同種異體移植物是否存活.結果 對照組移植物均齣現瞭明顯的急性排斥反應.兩實驗組術後7天組織學變化較對照組明顯為輕,14天時喉移植物的組織結構保持完整.實驗Ⅱ組14天時有2隻動物組織檢查顯示粘膜及粘膜下層充血水腫和以多形覈白細胞為主的炎性細胞浸潤,粘膜上皮壞死脫落,潰瘍形成和腺體壞死消失等感染徵象,另1隻則僅錶現為輕度的排斥反應.結論 CsA能有效阻止受體急性排斥反應的髮生,其功效與給藥途徑和劑量關繫密切,大劑量CsA的應用可能會增加受體的感染機會.
목적 연구배포소A(CsA)대동충이체후이식물배척반응적억제공효、상응적조직학개변이급여CsA응용제량지간적관계.방법 공체위봉폐군SD대서,수체위Wistar대서,장수체분위대조조화량개실험조,실험Ⅰ조화Ⅱ조매천분별경복강주사CsA 15 mg·kg-1·d-1화25mg·kg-1·d-1,련속2주.통과수체국부해부소견화조직학검사래판정동충이체이식물시부존활.결과 대조조이식물균출현료명현적급성배척반응.량실험조술후7천조직학변화교대조조명현위경,14천시후이식물적조직결구보지완정.실험Ⅱ조14천시유2지동물조직검사현시점막급점막하층충혈수종화이다형핵백세포위주적염성세포침윤,점막상피배사탈락,궤양형성화선체배사소실등감염정상,령1지칙부표현위경도적배척반응.결론 CsA능유효조지수체급성배척반응적발생,기공효여급약도경화제량관계밀절,대제량CsA적응용가능회증가수체적감염궤회.
Objective To study the inhibitory effects of cyclosporine A (CsA) on the laryngeal allograft rejection and corresponding histological changes,and the relation to the CsA dose.Methods The Wistar rats as recipients were divided into control group(without CsA)and two experimental groups:group Ⅰ and group Ⅱ receiving CsA 15 mg/kg and 25 mg/kg daily for 2 weeks respectively.Topographic anatomically and histologically,the survival of the laryngeal allografts was evaluated.Results Obvious histologic evidence of acute rejection of the laryngeal allografts was found in the control group.In the two experimental groups,the histological changes was obviously lessened 7 days after operation as compared with those in the control group,and the tissue structure of the laryngeal allografts was kept intact 14 days after transplantation.In the group Ⅱ,2 allografts histologically showed an increase in inflammatory cells in the lamina propria,predominantly polymorphonuclear leukocytes,with associated edema.surface epithelium and minor salivary gland acini were damaged,with focal ulceration and acinar micro-abscesses.The other case only exhibited slight rejection.Conclusion CsA can effectively prevent rejection of laryngeal allografts and block the development of injury of allografts from SD rat donors to Wistar recipients.The dosage and the pattern of administration of CsA may be closely related to its efficacy.The increased infection rate of the allografts may possibly be contributed to the higher CsA doses employed.
