CAJ | 학술논문

目的评价作为骨修复材料的无机活性元素骨组织工程支架材料的细胞亲和性.方法测试无机活性元素骨组织工程支架材料的比表面积、孔径分布、孔隙率、吸水率.将体外培养扩增并经过流式细胞仪鉴定的人骨髓间充质干细胞(MSCs)与无机活性元素骨组织工程支架材料复合培养,以单纯接种培养MSCs为对照组,噻唑蓝(MTT)法检测材料对细胞增殖的影响,扫描电镜观察材料表面细胞生长状况.结果无机活性元素骨组织工程支架材料比表面积为210 m2/g,平均微孔直径为6 nm,孔隙率90%,有34%的吸水率.MTT法显示培养至第7天时材料组细胞增殖与对照组有显著性差异(P＜0.05),扫描电镜下见材料表面细胞生长状况良好.结论无机活性元素骨组织工程支架材料具有良好的细胞亲和性,是一种较为合适的极具潜力的骨修复材料.
목적 평개작위골수복재료적무궤활성원소골조직공정지가재료적세포친화성.방법 측시무궤활성원소골조직공정지가재료적비표면적、공경분포、공극솔、흡수솔.장체외배양확증병경과류식세포의감정적인골수간충질간세포(MSCs)여무궤활성원소골조직공정지가재료복합배양,이단순접충배양MSCs위대조조,새서람(MTT)법검측재료대세포증식적영향,소묘전경관찰재료표면세포생장상황.결과 무궤활성원소골조직공정지가재료비표면적위210 m2/g,평균미공직경위6 nm,공극솔90%,유34%적흡수솔.MTT법현시배양지제7천시재료조세포증식여대조조유현저성차이(P＜0.05),소묘전경하견재료표면세포생장상황량호.결론 무궤활성원소골조직공정지가재료구유량호적세포친화성,시일충교위합괄적겁구잠력적골수복재료.
Objective To study the cellular affinity of the inorganic active element bone scaffold materials. Methods The specific surface area, pore size distribution, porous ratio, permeability ratio of the inorganic active element bone scaffold materials were evaluated in vitro. Human marrow mesenchymal stem cells(MSCs) were cultured and identified by the flow cytometry, the third passage of the cells were used to culture with scaffold materials, to compare with the cells cultured with pure fetal calf serum as the control group. MTT assay was used to examine the effects of scaffold materials on MSCs viability, the growth of cells was observed by scanning electron microscope (SEM). Results The specific surface area of the inorganic active element bone scaffold materials was 210 m2/g, mean pore size was 6 nm, porous ratio was 90 %, and permeability ratio was34 %. MTT assay showed significant difference in the number of cells between the scaffold materials and the control group (P ＜ 0.05 ) on the seventh day, MSCs co-cultured with scaffold materials exhibited good growth as observed under SEM. Conclusion The inorganic active element bone scaffold materials possess preferable cellular affinity, which could be an overall-developed and high potential scaffold material of bone tissue engineering.