中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2004年
24期
5189-5191
,共3页
舒茂琴%何作云%何国祥%宋治远%王国超
舒茂琴%何作雲%何國祥%宋治遠%王國超
서무금%하작운%하국상%송치원%왕국초
链脲佐菌素/副作用%动脉粥样硬化/病因学%脂类/血液
鏈脲佐菌素/副作用%動脈粥樣硬化/病因學%脂類/血液
련뇨좌균소/부작용%동맥죽양경화/병인학%지류/혈액
背景:apoE基因敲除或apoE2转基因小鼠是研究高三酰甘油血症与动脉粥样硬化(atherosclerosis AS)较好模型,但缺乏方便实用的动物模型.目的:探讨链脲佐菌素(streptozotocin,STZ)和饮食结构对新西兰大白兔血脂组分及AS的作用,以寻找方便实用的研究高三酰甘油血症与AS动物模型.设计:随机对照的实验研究.地点、材料和干预:实验在解放军第三军医大学西南医院中心实验室完成.实验选用健康雄性新西兰大白兔.采用随机抽签法分为4组:标准兔小颗粒饲养(SC)组;高胆固醇饲料饲养(HC)组;STZ处理后饲养标准兔小颗粒(STZ+SC)组;STZ处理后饲养高胆固醇饲料(STZ+HC)组.比较STZ和高胆固醇饲料对新西大白兔血脂组分和AS病变的作用,并分析AS病变局部清道夫受体A(scavenger receptor,SRA)表达与泡沫细胞分布的关系.主要观察指标:观察各组大白兔血浆脂蛋白和载脂蛋白水平和主动脉内膜病理变化,并分析血管内膜粥样硬化斑块局部SRA表达与泡沫细胞分布的关系.结果:STZ组大白兔血浆三酰甘油[(2.59±0.64)mmol/L]较SC组[(1.12±0.34)mmol/L]轻-中度升高(P＜0.05)、HDL-C降低及大动脉AS病变;STZ+HC组大白兔的血浆总胆固醇水平[(14.18±2.72)mmol/L]与HC组[(13.74±1.43)mmol/L]相似,而前者的血浆三酰甘油水平[(3.84±0.73)mmol/L]显著高于后者[(1.50±0.35)mmol/L,P＜0.05];STZ+HC组大白兔的AS病变最严重,单纯STZ能导致AS形成,但其AS的严重程度略轻于HC组.STZ所致的血脂紊乱与大多数冠心病患者相似,其AS病变局部的泡沫细胞绝大多数来源于单核巨噬细胞,SRA基因及蛋白质表达与巨噬细胞分布相同,其表达量与AS病变程度一致.结论:STZ处理并饲养普通饲料复制出的高三酰甘油血症和AS动物模型是较好的模型.
揹景:apoE基因敲除或apoE2轉基因小鼠是研究高三酰甘油血癥與動脈粥樣硬化(atherosclerosis AS)較好模型,但缺乏方便實用的動物模型.目的:探討鏈脲佐菌素(streptozotocin,STZ)和飲食結構對新西蘭大白兔血脂組分及AS的作用,以尋找方便實用的研究高三酰甘油血癥與AS動物模型.設計:隨機對照的實驗研究.地點、材料和榦預:實驗在解放軍第三軍醫大學西南醫院中心實驗室完成.實驗選用健康雄性新西蘭大白兔.採用隨機抽籤法分為4組:標準兔小顆粒飼養(SC)組;高膽固醇飼料飼養(HC)組;STZ處理後飼養標準兔小顆粒(STZ+SC)組;STZ處理後飼養高膽固醇飼料(STZ+HC)組.比較STZ和高膽固醇飼料對新西大白兔血脂組分和AS病變的作用,併分析AS病變跼部清道伕受體A(scavenger receptor,SRA)錶達與泡沫細胞分佈的關繫.主要觀察指標:觀察各組大白兔血漿脂蛋白和載脂蛋白水平和主動脈內膜病理變化,併分析血管內膜粥樣硬化斑塊跼部SRA錶達與泡沫細胞分佈的關繫.結果:STZ組大白兔血漿三酰甘油[(2.59±0.64)mmol/L]較SC組[(1.12±0.34)mmol/L]輕-中度升高(P＜0.05)、HDL-C降低及大動脈AS病變;STZ+HC組大白兔的血漿總膽固醇水平[(14.18±2.72)mmol/L]與HC組[(13.74±1.43)mmol/L]相似,而前者的血漿三酰甘油水平[(3.84±0.73)mmol/L]顯著高于後者[(1.50±0.35)mmol/L,P＜0.05];STZ+HC組大白兔的AS病變最嚴重,單純STZ能導緻AS形成,但其AS的嚴重程度略輕于HC組.STZ所緻的血脂紊亂與大多數冠心病患者相似,其AS病變跼部的泡沫細胞絕大多數來源于單覈巨噬細胞,SRA基因及蛋白質錶達與巨噬細胞分佈相同,其錶達量與AS病變程度一緻.結論:STZ處理併飼養普通飼料複製齣的高三酰甘油血癥和AS動物模型是較好的模型.
배경:apoE기인고제혹apoE2전기인소서시연구고삼선감유혈증여동맥죽양경화(atherosclerosis AS)교호모형,단결핍방편실용적동물모형.목적:탐토련뇨좌균소(streptozotocin,STZ)화음식결구대신서란대백토혈지조분급AS적작용,이심조방편실용적연구고삼선감유혈증여AS동물모형.설계:수궤대조적실험연구.지점、재료화간예:실험재해방군제삼군의대학서남의원중심실험실완성.실험선용건강웅성신서란대백토.채용수궤추첨법분위4조:표준토소과립사양(SC)조;고담고순사료사양(HC)조;STZ처리후사양표준토소과립(STZ+SC)조;STZ처리후사양고담고순사료(STZ+HC)조.비교STZ화고담고순사료대신서대백토혈지조분화AS병변적작용,병분석AS병변국부청도부수체A(scavenger receptor,SRA)표체여포말세포분포적관계.주요관찰지표:관찰각조대백토혈장지단백화재지단백수평화주동맥내막병리변화,병분석혈관내막죽양경화반괴국부SRA표체여포말세포분포적관계.결과:STZ조대백토혈장삼선감유[(2.59±0.64)mmol/L]교SC조[(1.12±0.34)mmol/L]경-중도승고(P＜0.05)、HDL-C강저급대동맥AS병변;STZ+HC조대백토적혈장총담고순수평[(14.18±2.72)mmol/L]여HC조[(13.74±1.43)mmol/L]상사,이전자적혈장삼선감유수평[(3.84±0.73)mmol/L]현저고우후자[(1.50±0.35)mmol/L,P＜0.05];STZ+HC조대백토적AS병변최엄중,단순STZ능도치AS형성,단기AS적엄중정도략경우HC조.STZ소치적혈지문란여대다수관심병환자상사,기AS병변국부적포말세포절대다수래원우단핵거서세포,SRA기인급단백질표체여거서세포분포상동,기표체량여AS병변정도일치.결론:STZ처리병사양보통사료복제출적고삼선감유혈증화AS동물모형시교호적모형.
BACKGROUND: The apoE gene knock-out mice or apoE2-transgeneic mice are better models for studying hypertriglyceridemia and atherosclerosis (AS) ,but convenientand practical animal models are still lacking.OBJECTIVE: To probe into the effects of streptozotocin(STZ) and dietary structure on the blood lipid contents and AS lesions in the New Zealand White(NZW) rabbits, and create a suitable and useful animal model for the study of hypertriglyceridemia and AS.DESIGN: Randomized and controlled study.SETTING, MATERIALS and INTERVENTIONS: This experiment was carried out in the Central Laboratory of Southwest Hospital, Third Military Medical University of Chinese PLA. Male healthy NZW rabbits were selected and divided into 4 groups with the method of random sampling: standard rabbit small particles breeding group (SC group); high cholesterol breeding group(HC group); rabbit small particles breeding after STZ treatment group (STZ + SC group); high cholesterol breeding after STZ treatment group (STZ + HC group) . Effects of STZ and high cholesterol feed on blood lipid changes and AS lesion of NZW rabbits were compared and the relationship of AS lesion partial A scavenger receptor(SRA) expression and distribution of foam cells was analyzed.MAIN OUTCOME MEASURES: The concentrations of plasma lipoprotein and apolipoprotein and pharmacological changes of aortic tunica intima of rabbits were observed. Besides, relationship between the partial SRA expression of AS plaques and distribution of foam cells were analyzed.RESULTS: Triacylglycerol(TG) in plasm of rabbits in the STZ group [ (2.59 ± 0. 64) mmol/L] was mildly-medially higher than that of the SC group [(1.12±0. 34) mmol/L] (P ＜0.05); The high density lipoprotein cholesterol(HDL-C) decreased and large artery AS lesion occurred; In the STZ + HC group, plasm total cholesterol level of white rabbits[ (14.18 ±2.72) mmol/L]was similar to that of HC group[ (13.74±1.43) mmol/L], while plasm triacylglycerol level[ (3.84 ± 0.73) mmol/L] of the former group was much higher than that of the latter group[ (1.50 ± 0.35) mmol/L, P ＜0.05 ]; AS lesion of rabbits in STZ + HC group was most serious, and STZ alone could cause AS lesion, but its AS severity was a little milder than that of HC group. The blood lipid disorder caused by STZ was similar to that of most clinical patients with coronary heart disease, in whom most of the foam cells in the AS lesions came from monocyte-macrophage cells. Gene and protein expressions of SAR were the same as the histocyte distribution, its expression dose was in accordance with the degree of AS lesion.CONCLUSION: Animal models in hypertriglyceridemia and AS lesion treated with STZ and fed with normal feed are better for the study of hypertriglyceridemia and AS.
